α-烯基-β-环糊精毛细管电色谱整体柱的制备与评价  

Preparation and Evaluation of α-Alkenyl-β-cyclodextrin Monolithic Column for Capillary Electrochromatography

作　　者：李英杰[1] 丁爽[1] 吕仁江[1] 丁会敏[1] 邓磊[1] 侯学功[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《分析测试学报》2011年第11期1290-1294,共5页Journal of Instrumental Analysis

摘　　要：将烯丙基缩水甘油醚(AGE)和β-环糊精在碱性条件下作用得到带有α烯基的环糊精衍生物——4-(3-烯丙氧-2-羟基)丙氧基-β-环糊精(PCD),利用这种衍生物和甲基丙烯酸甘油酯(GMA)为功能单体,在毛细管中通过原位聚合反应,一步法制备得到了新型β-环糊精聚合物毛细管电色谱手性整体柱。在毛细管电色谱(CEC)模式下,应用制备的手性整体柱对3种氨基酸和手性药物愈创甘油醚对映体进行拆分,并考察了有机调节剂、缓冲溶液pH值及柱温对拆分效果的影响。结果显示,在优化色谱条件下,4种对映体均得到基线分离,分析时间小于8 min。所制备的新型手性整体柱具有较强的手性拆分能力,且重现性好、操作简单。In alkaline conditions, 4-（ 3-acryloxy-2-hydroxy ） propyloxy-β-cyclodextrin（ PCD ） was synthesized based on the reaction of 1-alklyloxy-2, 3-epoxy propane （also named as allyl glycidyl ether, AGE） and β-cyclodextrin（β-CD）. A new type β-cyclodextrin polymer chiral monolithic column for CEC was prepared by using the in-situ polymerization of PCD and GMA as functional monomers in the capillary tube. The prepared monolithic column was evaluated by separating racemic mixtures of three amino acids and a chial drug （guguaifenesin） in capillary electrochromatography mode. The influences of organic modifier, buffer pH value and temperature on separation were investigated. ibility and easy operation.

关 键 词：整体柱 毛细管电色谱 烯丙基缩水甘油醚β-环糊精 DL-氨基酸 对映体分离 

分 类 号：O657.8[理学—分析化学] Q517[理学—化学]