超抗原金黄色葡萄球菌肠毒素A对活化T细胞端粒长度影响及其相关机制研究  

Effect of SEA on Telomere Length of T cells in cord blood

作　　者：秦雅楠[1] 王冠明[1] 张鹏[1] 林晨[1] 高永鹏[1] 田红霞[1] 李扬秋[2] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广州市510632 [2]暨南大学医学院血液病研究所,广州市510632

出　　处：《实用医学杂志》2011年第22期4015-4017,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金项目(编号:06025169);广州市科技支撑计划项目(编号:2009Z1-E161)

摘　　要：目的:研究超抗原金黄色葡萄球菌肠毒素A(SEA)对T细胞端粒长度的作用及机制。方法:SEA、K562细胞、SEA联合K562细胞以及抗CD3单克隆抗体分别刺激脐带血单核细胞,SYBR GreenⅠ荧光定量PCR检测端粒、hTERT及IL-7R表达。结果:SEA组、K562细胞组、SEA联合K562细胞组及抗CD3单抗组活化T细胞的端粒相对长度比值为1.63±0.08、1.06±0.06、3.24±0.61、1.37±0.07;hTERT的表达差异倍数为1.88±0.07、1.95±0.05、3.93±0.23、1.30±0.06;IL-7R表达差异倍数为6.61±0.52、12.81±0.34、17.42±0.50、4.87±0.66;SEA联合K562细胞组均明显高于其他3组(P<0.01),SEA组对端粒长度影响大于单纯K562细胞组。结论:超抗原SEA可以直接刺激T细胞的端粒酶活性及上调表达IL-7R,联合特异性抗原K562细胞株诱导T细胞活化上调效果更明显。Objective To study the effect and mechanism of SEA on telomere of T cells. Methods Real- time PCR with SYBR Green I technique was used for detecting the expression level of telomere, hTERT and IL-7R in cord blood mononuclear cells, which were induced by K562 cells, SEA or both of K562 cells and SEA, the anti- CD3 antibodies respectively. Results In four groups of the SEA, K562 cells, both of SEA and K562 cells,the anti- CD3 antibodies, the expression level of telomere respectively were （1.63 ± 0.08）, （1.06 ± 0.06）, （3.24 ± 0.61 ） （ 1.37 ± 0.07 ） ;The expression level of hTERT were （ 1.88 ± 0.07 ）, （ 1.95 ± 0.05 ）, （3.93 ± 0.23 ） （ 1.30 ± 0.06） ;The expression level of IL-7R were（6.61 ± 0.52）, （ 12.81 ± 0.34）, （ 17.42 ± 0.50）, （4.87 ± 0.66） ;These results showed that the expression level of telomere,IL-7R and hTERT induced by both SEA and K562 were significantly higher than that of the other three groups （P 〈 0.01 ）, and the effect of SEA on the length of telomere was higher than K562 cells. Conclusion The superantigen（SEA） could enhance the activity of telomerase and the expression of IL-7R, particularly having much more effect combined with K562 cells in vitro.

关 键 词：白血病 髓系 慢性 BCR-ABLB阳性 肠毒素类 端粒长度 IL-7R hTERT 

分 类 号：R392[医药卫生—免疫学]