肾上腺素α_2受体调控MAPK通路对VILI大鼠肺部炎症的影响  

Effects of alpha-2 adrenoceptor-regulated MAPK pathway on the lung inflammatory response in a rat model of ventilator-induced lung injury

作　　者：吕婧[1] 忽新刚[1] 黎镇赐[1] 于霖[1] 丁宁[1] 许立新[1] 佘守章[1] 

机构地区：[1]广州医学院附属广州市第一人民医院,510000

出　　处：《实用医学杂志》2011年第22期4037-4039,共3页The Journal of Practical Medicine

基　　金：广州市医药卫生科技重点资助项目(编号:2009-ZDi-02)

摘　　要：目的:研究肾上腺素α_2受体调控的炎症信号传导MAPK通路对呼吸机所致肺损伤大鼠肺部炎症反应的影响。方法:30只SPF级雄性SD大鼠随机均分成5组:常规潮气量通气组(C,8 mL/kg,呼吸频率90次/min),大潮气量通气组(H,20 mL/kg,呼吸频率50次/min),大潮气量通气盐酸右旋美托咪啶处理组(D,参数设置同H组),大潮气量通气育亨宾处理组(Y,参数设置同H组),大潮气量通气+盐酸右旋美托咪啶+育亨宾处理组(D+Y,参数设置同H组)。观察肺部病理改变,ELISA法检测支气管肺泡灌洗液(BALF)中的肿瘤坏死因子α、IL-1β、IL-6和IL-10,Western Blot方法检测各组肺组织中促分裂原活化蛋白激酶及其和磷酸化水平。结果:和C组相比,H组、D组、Y组和D+Y组大鼠肺组织病理改变,BALF中的TNF-α、IL-1β、IL-6、IL-10和MIP-2,以及肺部磷酸化ERK1/2均有明显增高。H组、Y组和D+Y组相比,D组肺组织和BALF中各项指标均显著降低。结论:α_2受体激动剂能通过调控MAPK通路显著减轻VILI所造成的肺部炎症反应,提示α_2受体在VILI所造成的肺部炎症反应中起到重要作用。Objective To elucidate the effect of alpha-2 adrenoceptor-regulated inflammatory signal pathway MAPK on the lung inflammatory response in a rat model of ventilator-induced lung injury. Methods Thirty SPF adult male Sprague-Dawley rats were randomly divided into five groups （n = 6 per group） ： standard ventilation group （C）, high-tidal volume ventilation group （H）, high-tidal volume ventilation plus dexmedetomidine （D）, high-tidal volume ventilation plus yohimbine（Y）, high-tidal volume ventilation plus dexmedetomidine and yohimbine （D＋Y）.The pulmonary pathologic changes were observed. The levels of tumor necrosis factor α, interleukin （IL-1 1β, IL-6 and IL-10） in the BALF were determined by ELISA assay. The expressions of ERKI/2 and p-ERK1/2 were measured by Western blot method. Results Compared with Group C, Group H, D, Y, and D＋Y had a significant pulmonary pathologic changes （P 〈 0.01）. The expression levels of TNF-α, IL-1β, IL-6 and IL-10 in BALF in Group H, D, Y and D＋Y were significantly higher than those in Group C. The indices of lung tissue homogenization and BALF in Group D were significantly lower than those in Group H,D,Y,and D＋Y. Conclusion Alpha-2 adrenoceptor agonist significantly attenuated the inflammation response of lung underwent injurious ventilation via regulating the MAPK pathway, indicating that alpha-2 adrenoceptor plays an important role in the pulmonary inflammation of VILI.

关 键 词：肺炎 呼吸机相关性 盐酸右旋美托咪啶 育亨宾 炎症 细胞外信号调控蛋白激酶1/2 

分 类 号：R563.1[医药卫生—呼吸系统]