多功能灵敏固相时间分辨荧光免疫分析仪设计  

Design of a new solid-phase time-resolved fluorescence immunoassay analyzer

作　　者：宋克非[1] 张佩杰[1] 

机构地区：[1]中国科学院长春光学精密机械与物理研究所,长春130033

出　　处：《仪器仪表学报》2011年第11期2609-2615,共7页Chinese Journal of Scientific Instrument

摘　　要：针对固相时间分辨荧光光谱的测量,设计出一种全新的固相时间分辨荧光免疫分析系统。使用氮分子激光器作为激发光源,采用积分球和单色仪相结合的荧光收集结构,使杂散光对样品荧光的影响降到最低;用光电倍增管进行光电转换,在500～700 nm范围实现了高分辨荧光光谱测量;利用数字方式实现取样积分功能,提高了系统的信噪比。系统可实现荧光寿命、时间分辨荧光光谱、物质浓度的自动测量,仪器的检测灵敏度可达10-12 mol/L,线性范围为10-12～10-9 mol/L,稳定性相对误差小于3%,荧光光谱分辨为0.5 nm。A new solid-phase time-resolved fluorescence immunoassay analysis system has been designed for solid-phase time-resolved fluorescence spectroscopy measurements.The device uses nitrogen molecular laser as the excitation light source and employs integrating sphere combined with grating monochromator as the fluorescence collecting subsystem to minimize the influences of stray light on the sample fluorescence.Photomultiplier tube is used to convert the sampled optic signal to electronic signal and high resolution measurements of fluorescence spectrum in the range of 500～700 nm are implemented.The converted electrical signals are sampled and integrated digitally with a single chip micro-computer,which improves the signal to noise ratio of the received signal.The instrument can complete fluorescence lifetime measurement,time-resolved fluorescence spectroscopy measurement and substance concentration measurement.Experimental results show that the measurement sensitivity is up to 10-12 mol/L,linearity range is 10-12～10-9 mol/L,relative stability error is lass than 3% and the measurement resolution is 0.5 nm.

关 键 词：固相时间分辨 荧光免疫分析系统 光栅单色仪 积分球 

分 类 号：TP216[自动化与计算机技术—检测技术与自动化装置]