HPLC测定新疆药桑叶中绿原酸、芦丁、异槲皮苷和槲皮素  

Determination of Chlorogenic Acid,Rutin,Isoquercitrin and Quercetin in Drug Mulberry Leaves of Xinjiang by HPLC

作　　者：于小蓉[1] 孙莲[1] 孟磊[2] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830054 [2]新疆医科大学分析测试中心,乌鲁木齐830054

出　　处：《中国现代应用药学》2011年第11期1051-1054,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立新疆药桑叶中绿原酸、芦丁、异槲皮苷和槲皮素的高效液相色谱测定方法。方法采用BDS C18色谱柱(4.6 mm×250 mm,5μm),柱温:25℃,流动相:乙腈(A)-0.01 mol.L-1醋酸铵溶液(100 mL中含0.2 mL三乙胺,用冰醋酸调pH至4.8)(B),梯度洗脱[0 min,A-B(8∶92);30 min,A-B(30∶70)],流速:1.0 mL.min-1,检测波长350 nm。结果绿原酸在1.052 0μg～5.067 9μg(r=0.999 3)、芦丁在1.029 4μg～5.031 7μg(r=0.999 8)、异槲皮苷在1.076 8～5.065 5μg(r=0.999 0)、槲皮素在1.075 0μg～5.055 0μg(r=0.999 1)内线性关系良好。绿原酸、芦丁、异槲皮苷和槲皮素的平均回收率分别为102.2%(RSD=1.3%)、104.6%(RSD=0.9%)、104.0%(RSD=1.9%)、104.7%(RSD=1.2%)。检出限(S/N=3)分别为0.160,0.081,0.033,0.640μg.mL-1。结论此法操作简便、快速、灵敏、准确,样品处理简便易行,适于同时测定新疆药桑叶中绿原酸、芦丁、异槲皮苷和槲皮素。OBJECTIVE An HPLC method was established for the simultaneous determination of Chlorogenic Acid,Rutin,Isoquercitrin and Quercetin in drug Mulberry Leaves of Xinjiang.METHODS The chromatographic separation was carried out on a Yilite Hypersil BDS（4.6 mm × 250 mm,5 μm） with a mobile phase composed of acetonitrile（A） and 0.01 mol·L-1 ammonium acetate（pH=4.8）（B） with gradient elution [0 min,A-B（8∶92）;30 min,A-B（30∶70）].The flow rate was 1.0 mL·min-1,the detection wavelength was set at 350 nm and the column temperature was 25 oC.RESULTS The linear ranges of Chlorogenic acid,Rutin,Isoquercitrin,and Quercetin were between 1.052 0-5.067 9 μg（r=0.999 3）,1.029 4-5.031 7 μg（r=0.999 8）,1.076 8-5.065 5 μg（r=0.999 0） and 1.075 0-5.055 0 μg（r=0.999 1）,respectively.The average recoveries of Chlorogenic Acid,Rutin,Isoquercitrin and Quercetin were 102.2%（RSD=1.3%）,104.6%（RSD=0.9%）,104.0%（RSD=1.9%）,104.7%（RSD=1.2%）.The detection limits were 0.160,0.081,0.033,0.640 μg·mL-1（S/N=3）,respectively.CONCLUSION The method is simple,rapid,sensitive and accurate.Therefore it is applicable for the determination of Chlorogenic Acid,Rutin,Isoquercitrin and Quercetin in drug Mulberry Leaves of Xinjiang.

关 键 词：药桑叶 绿原酸 芦丁 异槲皮苷 槲皮素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]