发育期六溴环十二烷染毒对雌性大鼠脑组织中乙酰胆碱含量及其相关酶活力的影响  

Effects of Hexabromocyclododecane on Acetylcholine Contents and Related Enzymes Activities of Brain in Female Rats in Growth and Development Period

作　　者：刘芳[1] 冀秀玲[1] 唐蔚[1] 骆庆和[2] 赵文娟[2] 殷明[2] 

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]上海交通大学药学院

出　　处：《环境与健康杂志》2011年第11期947-949,共3页Journal of Environment and Health

基　　金：国家自然科学基金(20907007);国家自然科学基金(30970685);教育部霍英东基金优选项目(114044);上海市重点学科开放基金(B604);上海市科委基金(09140901900)

摘　　要：目的研究环境暴露水平的六溴环十二烷(HBCD)对发育期大鼠的胆碱能神经系统的神经毒性作用。方法将35只3日龄清洁级Sprague-Dawley(SD)雌性大鼠随机分为5组,分别为对照(花生油)组和10、50、100、300μg/kg HBCD染毒组,每组7只。采用灌胃方式进行染毒,染毒容量为1 ml/kg,连续染毒42 d。测定脑组织中乙酰胆碱(Ach)的含量及乙酰胆碱酯酶(AchE)和乙酰胆碱转移酶(ChAT)的活力。结果与对照组比较,100、300μg/kg HBCD染毒组雌性大鼠脑组织中Ach的含量降低,300μg/kg HBCD染毒组脑组织中AchE活力和10μg/kg HBCD染毒组脑组织中ChAT活力升高,差异均有统计学意义(P<0.05)。结论 HBCD染毒可对脑发育期大鼠的胆碱能系统产生损伤作用,进而对神经发育产生影响。Objective To know the neurotoxicity of hexabromocyclododecane （HBCD） to female rats in growth and development period. Methods Sprague-Dawley rats of 3-days old were exposed to different doses （10,50,100 and 300 μg/kg） of HBCD through garage with the volume of 1 ml/kg, for 42 consecutive days. The alkaline hydroxylamine eolorimetric method and ASCh-DTNB method were used to determine the acetylcholine （Ach） contents and activities of cholinesterase （AchE）, choline acetyltransferase （CHAT）. Results Compared with the control, the Ach content decreased in 100 and 300 μg/kg of HBCD groups, and AchE activity increased in 10-300 μg/kg exposure doses with a dose dependent manner. Conclusion HBCD exposure may damage the cholinergic system of female rats in growth and development period, which may produce the adverse effects on the development of central nervous system.

关 键 词：六溴环十二烷 乙酰胆碱 乙酰胆碱酯酶 乙酰胆碱转移酶 发育期 大鼠 

分 类 号：R994.6[医药卫生—毒理学]