BDE-153暴露对大鼠中枢胆碱能系统关键酶活力及细胞内钙离子浓度的影响  

Effects of BDE-153 Exposure on Rats' Cortex Cholinergic System Key Enzymes and Intracellular Calcium Concentration

作　　者：张红梅[1] 聂继盛[2] 吴智文[1] 刘晓虎[1] 王亮[1] 康慧杰[1] 牛侨[2] 

机构地区：[1]山西医科大学公共卫生学院环卫教研室,山西太原030001 [2]山西医科大学公共卫生学院劳卫教研室

出　　处：《环境与健康杂志》2011年第11期950-953,共4页Journal of Environment and Health

基　　金：国家自然科学基金(81001258)

摘　　要：目的研究哺乳期BDE-153暴露对大鼠成年后大脑皮质胆碱能系统关键酶的活力及细胞内Ca2+浓度的影响。方法将40只出生第4天的雄性清洁级SD大鼠按体重随机分成4组,分别为溶剂对照(橄榄油)组和低(1 mg/kg)、中(5 mg/kg)、高剂量(10 mg/kg)BDE-153染毒组,每组10只。在仔鼠出生第10天,采用一次性腹腔注射方式进行染毒,染毒容量为0.01 ml/g。染毒两个月后,采用流式细胞仪测定大脑皮质细胞内Ca2+的浓度,采用比色法测定皮质乙酰胆碱转移酶(ChAT)和乙酰胆碱酯酶(TchE)的活力。结果与溶剂对照组比较,各剂量BDE-153染毒组大鼠大脑皮质神经细胞内Ca2+浓度均降低,差异有统计学意义(P<0.05);仅高剂量BDE-153染毒组皮质组织内ChAT和TchE活力降低,差异均有统计学意义(P<0.05)。结论哺乳期BDE-153暴露可抑制成年大鼠大脑皮质胆碱能系统的功能,使细胞内Ca2+浓度降低,这可能是BDE-153神经毒性的主要机制之一。Objective To study the effects of lactation BDE-153 exposure on the activity of key enzymes in rat cortex eholinergic system and intracellular calcium （Ca2＋） concentration. Methods Forty newborn clean male SD rats were randomly divided into four groups at postnatal clay 4 （PND4） according to their body weights, 10 in each group, 1,5,10 mg/kg of BDE- 153 group and olive oil solvent control group. At PND10,the rats were administrated BDE-153 olive oil solution by intraperitoneal injection at 0.01 ml/g body weight for one time. Two months later,rats were anesthetized and followed the cortex separation for the intraceiiular Ca2＋ concentration determination by flow cytometry and for the activities of choline acetyhransferase （CHAT） and true cholinesterase （TchE） detection by colorimetric methods. Results Compared with the control group,cortical intracellular Ca2＋ concentration were significantly decreased in all the BDE-153 treated groups（P〈0.05）,while the cortical ChAT and TchE activities were significantly declined in the 10 mg/kg BDE-153 treated group. Conclusion Lactation BDE-153 exposure can inhibit the cholinergic system function of rats＇ cortex,and reduce the intracellular Ca2＋ concentration, which may be one of the mechanisms of BDE-153 neurotoxicity.

关 键 词：多溴联苯醚-153 乙酰胆碱转移酶 乙酰胆碱酯酶 细胞内钙离子浓度 

分 类 号：R994.6[医药卫生—毒理学]