产漆酶真菌筛选及其对PAHs污染土壤修复的初步研究  

SCREENING FOR LACCASE-PRODUCING FUNGUS AND ITS POTENTIAL IN REMEDIATION OF PAHS-CONTAMINATED SOIL

作　　者：潘澄[1,2] 茆婷[1] 吴宇澄[2] 申卫收[1] 钟文辉[1] 

机构地区：[1]南京师范大学环境科学与工程系,南京210097 [2]中国科学院土壤环境与污染修复重点实验室(南京土壤研究所),南京210008

出　　处：《土壤学报》2011年第6期1253-1259,共7页Acta Pedologica Sinica

基　　金：国际科学基金项目(IFSNo.C/4471-1);南京师范大学"211工程"重点学科建设项目(1843203623)

摘　　要：真菌漆酶可以高效转化多环芳烃(PAHs),因此,产漆酶真菌在PAHs污染土壤修复中极具应用前景。根据漆酶可将愈创木酚氧化为红色物质的特性,成功从土壤中筛选出一株能够分泌漆酶的真菌菌株F-1,初步鉴定该菌为疣孢漆斑菌(Myrothecium verrucaria)。通过Plackett-Burman试验对菌株F-1的产酶能力进行了分析,发现特定培养条件组合可将其酶活提高近300倍,达5628 U L-1,表明F-1的漆酶活性受到环境条件的显著影响。应用菌株F-1对PAHs污染土壤进行了初步修复研究,结果表明,接种F-1对菲、荧蒽、芘、苯并(a)蒽、屈、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,h)蒽、苯并(g,h,i)苝、茚苯(1,2,3-cd)芘等11种PAHs均有不同程度的降解,提示产漆酶真菌在PAHs污染土壤修复中的应用潜力。Fungal laccase can efficiently oxidize polycyclic aromatic hydrocarbons （PAHs）, suggesting the potential of laccase-producing fungi for application to remediation of PAHs-contaminated soil. Based on its feature of being capable of oxidizing guaiacol into something red, a strain of laccase-producing fungus, F-1, was isolated from soil. According the BLAST alignment of near full-length 18S rRNA gene sequence, this strain is closely related to Myrothecium verrucaria. Both single factor and Plackctt-Burman experiments were performed to assess F-1 ＇ s laccase-producing capacity. The laccase activity was found to be greatly increased by 2 orders of magnitude in specific culture medium, suggesting the significant effect of environment factors on laccase activity of F-1. Soil microcosms were set up with or without inoculation of F-1, and the PAHs contents were determined after 30-days of incubation. Results show that PhA, FluA, Pyr, BaA, Chr, BbF, BkF, BaP, DbA, BghiP and In[1,2,3-cd]P were degraded to varying extent in fungus inoculated microcosms, confirming the remedial potential of F-1 in PAHs-contaminated soil.

关 键 词：多环芳烃(PAHs) 土壤污染 真菌修复 漆酶 

分 类 号：X53[环境科学与工程—环境工程]