鹅催乳素受体多克隆抗体制备及组织表达分析  

Preparation of polycolonal antibody against goose prolactin receptor and the receptor expression in goose tissues

作　　者：邢光东[1,2] 杨廷桂[3] 段修军[3,4] 宦海琳[2] 闫俊书[2] 王根林[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]江苏省农业科学院畜牧研究所,江苏南京210014 [3]江苏畜牧兽医职业技术学院 [4]国家水禽基因库,江苏泰州225300

出　　处：《南京农业大学学报》2011年第6期105-108,共4页Journal of Nanjing Agricultural University

基　　金：江苏省农业自主创新资金项目(CX(09)638)

摘　　要：为深入研究鹅催乳素受体(PRLR)的功能,构建了含鹅PRLR胞外域编码序列exPRLR的原核表达质粒exPRLR-pET-28a(+),通过转化E.coli Roster建立了稳定的原核表达系统,在IPTG诱导下获得了PRLR胞外域的重组exPRLR。以重组exPRLR为抗原免疫家兔,获得了兔抗鹅exPRLR的抗血清。Western blot分析证明该抗血清可特异识别由原核生物表达的重组exPRLR。进一步分析成年母鹅的组织蛋白,发现成年鹅中可能至少存在3种相对分子质量不同的PRLR蛋白异形体。To study the functions of prolactin receptor（PRLR）in goose,we constructed a prokaryotic expression vector exPRLR-pET-28a（＋）that contains exPRLR,the coding sequences of the whole extracellular domain of goose PRLR.Transformed into host bacteria E.coli Roster and induced by IPTG,exPRLR-pET-28a（＋）expressed recombinant protein exPRLR.By immunizing rabbit with the recombinant protein,we obtained rabbit anti-goose exPRLR polycolonal serum.Western blot assay revealed that the anti-serum recognized the recombinant protein expressed by the transformed bacteria specifically.Analyzing the proteins isolated from adult female goose tissues with the anti-serum,we found that adult geese might have at least three isoforms of PRLR with different relative molecular mass.

关 键 词：鹅 催乳素受体 胞外域 原核表达 多克隆抗体 组织表达 

分 类 号：S835[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]