结核分枝杆菌CFP-10蛋白的克隆及表达  

Cloning and expression of CFP-10 protein of Mycobacterium tuberculosis

作　　者：张丹[1,2] 朱中元[1] 王海波[1] 

机构地区：[1]海南省农垦总医院,海南海口570311 [2]海南大学环境与植物保护学院,海南海口570208

出　　处：《中国热带医学》2011年第10期1180-1181,1216,共3页China Tropical Medicine

基　　金：海南省2008年度重点科技项目(No.080209)

摘　　要：目的通过构建结核分枝杆菌CFP-10蛋白表达载体,在大肠杆菌表达,为探索重组多肽在结核病血清学诊断的应用奠定前期实验基础。方法用PCR法从结核分枝杆菌H37Rv基因组中扩增出CFP-10基因片段,连接到表达载体PET30a上,在大肠杆菌中表达;组氨酸标签(His-Tag)镍柱层析纯化重组蛋白。结果构建了含CFP-10重组质粒的大肠杆菌工程菌,发现目的蛋白主要以可溶性蛋白形式存在。结论 CFP-10蛋白基因克隆入宿主菌中并表达成功。Objective To evaluate the potential of the antigens in serodiagnosis of TB,CFP-10 gene was cloned and expressed in Escherichia coli.Methods The gene coding CFP10 protein was amplified by PCR from genome of Mycobacterium tuberculosis H37Rv,and then inserted into expression vector PET30a and expressed fusion proteins CFP10 in E.coli BL21（DE3）.The recombinant proteins were purified by affinity chromatography.Results The target protein was expressed in E.coli after induction with IPTG.The solubility analysis showed that the recombinant protein existed as soluble protein.Conclusion The gene sequence of CFP10 was obtained and the protein was expressed in E.coli BL21.

关 键 词：结核分枝杆菌 CFP-10蛋白 表达 

分 类 号：R521[医药卫生—内科学]