不同临床期别宫颈鳞癌患者血清差异蛋白质的表达  

Differentially expressed proteins in serum of operation and non-operation cervical squamous cell carcinoma patients

作　　者：郭晓红[1] 梁卫江[1] 蔡思娜[1] 陈剑光[2] 张军一[1] 谢剑明[1] 吴一龙[2] 罗荣城[1] 

机构地区：[1]南方医科大学南方医院肿瘤中心,广东广州510515 [2]广东省人民医院肺癌研究所,广东广州510080

出　　处：《中国热带医学》2011年第10期1199-1201,1207,共4页China Tropical Medicine

基　　金：广东省科技计划项目(2007B030703004)

摘　　要：目的寻找不同临床期别宫颈鳞癌患者之间的血清差异蛋白质。方法采用表面增强激光解吸离子化飞行时间质谱仪(SELDI-TOF-MS)和弱阳离子结合芯片(CM10),检测宫颈鳞癌患者可手术治疗组(Ⅰa期、Ⅰb期、Ⅱa期)与不能手术治疗组(Ⅱb期、Ⅲa期、Ⅲb期、Ⅳ期)的血清蛋白质、以及血清鳞状细胞癌抗原(SCCA)≥4.5ng/ml组与SCCA<1.5ng/ml组之间有分类意义的差异蛋白质。结果相对分子质量在1002～18369范围内,共有43种蛋白质质谱峰值有显著性差异(P<0.05),其中质荷比(M/Z)为11523.83、11679.13、5841.076、7971.04、16109.71、15932.17的蛋白质,在宫颈癌可手术治疗组的含量显著低于不能手术治疗组(P<0.01)。SCCA≥4.5ng/ml组与SCCA<1.5ng/ml组之间获得相同的差异蛋白质。以单个差异蛋白质11679.13(M/Z)作为分类变量建立宫颈癌手术组与不能手术组的诊断树模型,分类正确率为93.18%(41/44)。结论宫颈癌早中期可手术治疗组与中晚期不能手术治疗组血清中存在差异蛋白质,SCCA组≥4.5ng/ml与SCCA<1.5ng/ml组之间存在相同的差异蛋白质;以单个差异蛋白质作为分类变量建立的诊断树模型,给临床分期提供重要的参考。Objective To find the differentially expressed proteins in early metaphase（operation group） and advanced stage（non-operation group） in the serum of patients with cervical squamous cell carcinoma.Methods Surface-enhanced laser desorption/ionization time of flight mass spectrometry（SELDI-TOF-MS） with weak cationic chips（CM10） was used to detect the serum samples of 44 patients with cervical squamous cell carcinoma.The differentially expressed proteins in the serum of the patients were identified.Differentially expressed proteins were detected in two groups based on SCCA（SCCA1.5ng/ml and SCCA≥4.5ng/ml）.Results The relative molecular mass was in the range of 1002～18369,43 mass spectra peak value in operation group and non-operation group showed significant difference（P0.05）,among which 6 proteins with mass/charge ratio of 11523.83、11679.13、5841.076、7971.04、16109.71 and 15932.17,respectively,showed lower expression in the serum of cervical cancer patients in operation group（P0.01）.The same differentially expressed proteins were detected in the two groups based on SCCA.The diagnostic model for cervical cancer was generated to discriminate operation group and non-operation group,based on 11 679.13（M/Z） protein with the correct classification of 93.18%（41/44）.Conclusion Differential protein expression existed in early metaphase（operation group） and advanced stage（non-operation group） in cervical carcinoma.The same proteins were detected in the two groups based on SCCA.The diagnostic model tree defined by simple differentially expressed protein provided important reference for clinical stage.

关 键 词：宫颈鳞状细胞癌 血清差异蛋白质 蛋白芯片-飞行质谱技术 鳞状细胞癌抗原 

分 类 号：R521.1[医药卫生—内科学]