2009年海南省甲型H1N1流感病毒核酸检测结果  

Analysis of H1N1 influenza virus nucleic acid testing in Hainan Province in 2009

作　　者：曾祥洁[1] 潘家兴[1] 马焱[1] 李臻[1] 李丹丹[1] 林春燕[1] 邱丽[1] 

机构地区：[1]海南省疾病预防控制中心,海南海口570203

出　　处：《中国热带医学》2011年第10期1253-1254,共2页China Tropical Medicine

基　　金：海南省自然科学基金立项课题(No.806100)

摘　　要：目的及时了解海南省2009年流感样病例及其密切接触者中甲型H1N1流感病毒感染情况。方法应用实时荧光定量PCR法对呼吸道鼻咽拭子标本中的流感病毒核酸进行定性检测。结果全年共检测哨点监测和爆发疫情标本1 148份,荧光定量PCR法检测结果:流感病毒A型核酸阳性为686份,B型核酸阳性为7份。流感样病例中流行型别以A型为主,占59.6%;A型中甲型H1N1流感病毒核酸阳性为500例,占72.9%(500/686),其中学生占84.8%(424/500)。10月-11月份达到流行高峰。随机抽取30份核酸阳性标本进行核酸序列测定,27份为甲型H1N1流感病毒。结论 2009年海南省流感样病例中甲型H1N1流感病毒感染主要以学生为主,开展流感病毒核酸检测,有助于尽早了解流感样病例中流感病毒感染情况,为及时了解甲型H1N1流感疫情的动态变化以及制定科学有效的甲型H1N1流感防控策略提供可靠的实验室依据。Objective To know in time H1N1 influenza virus infection situation in flu-like illness and their close contacts of Hainan Province in 2009.Method Real-time fluorescent quantitative（RT-PCR） method was applied to qualitatively detect the influenza virus nucleic acid of nasopharyngeal swab specimens of respiratory tract.Result Annual sentinel surveillance and outbreak specimens were 1 148,the fluorescent quantitative PCR results were below：686 specimens were tested with nucleic acid type A influenza virus positive,7 specimens were positive of B-type nucleic acid.The main population incidence type of influenza-like illness case was type A,accounting for 59.6%;among which there were 500 cases with H1N1 influenza virus nucleic acid positive,accounting for 72.9%（500/686）,in which students accounted for 84.8%（424/500）.October to November reached epidemic peak.30 nucleic acid-positive samples were randomly selected for sequencing,27 were H1N1 influenza virus.Conclusion The main infected people by influenza virus was students in Hainan Province in 2009.It＇s helpful to carry out influenza virus nucleic acid detection to know as soon as possible about influenza-like illness in influenza virus cases.It＇s necessary to know epidemic situation in time and to provide a reliable basis of sound and scientific prevention and control strategies formulation for H1N1 influenza virus.

关 键 词：甲型H1N1流感病毒 核酸检测 流感样病例 

分 类 号：R511.7[医药卫生—内科学]