盐胁迫下疏叶骆驼刺根基因表达差异及功能注释  

Gene expression diference and function annotation of Alhagi sparsifolia roots under salt stress

作　　者：赖黎丽[1,2] 赵洁[2] 张博[2] 王涛[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100193 [2]新疆农业大学草地资源与生态重点实验室,新疆乌鲁木齐830052

出　　处：《草原与草坪》2011年第5期1-6,共6页Grassland and Turf

基　　金：新疆维吾尔自治区高技术研究与发展计划项目"骆驼刺;苦马豆耐盐关键基因的克隆与转基因苜蓿培育"(200711101)资助

摘　　要：疏叶骆驼刺(Alhagi sparsifolia)是一种多年生豆科植物,它具有很强的耐冷、干旱和盐的能力。截型苜蓿(Medicago truncatula)是豆科的模式植物,所以本研究利用Affymetrix公司的MedicagoGenome Array与疏叶骆驼刺进行杂交,检测疏叶骆驼刺幼根220mmol/L NaCl溶液盐胁迫处理24h后的差异表达基因,以0h的样品为CK。结果表明:(1)在盐胁迫材料中检测到5 378个基因表达,占所有基因总数的8.8%,获得与之比较上调log2ratio值≥1的差异基因127个,下调ratio值≤-1的差异基因119个;(2)通过GO注释对这些差异表达基因进行初步分析表明:差异表达基因的上调和下调与疏叶骆驼刺幼根的耐盐性相关。这些都为疏叶骆驼刺耐盐机理的研究提供了有价值的信息。The expression of different genes in roots of Alhagi sparsifolia,under salt stress,were analyzed based on Medicago Genome Array hybridized with Alhagi sparsifolia.The roots had been exposed to 220 mmol/L NaCl for 24hand the untreated roots as control.The results showed that there were 5 378genes ex-pressed under salt stress（8.8%of the total genes）.And there were 127genes appeared to be up-regulated（with the ratio≥1）and 119appeared to be down-regulated（with the ratio≤-1）.These up and down regulated genes were related to salt tolerance based on GO annotation analyzing.Thses could provide some information to salt tolerance mechanism of Alhagi sparsifolia.

关 键 词：疏叶骆驼刺 基因芯片 盐胁迫 差异表达 

分 类 号：Q786[生物学—分子生物学]