扩增ccaR基因提高棒状链霉菌克拉维酸产量的研究  

Study on application of ccaR Streptomyces clavuligerus to increase clavulanic acid production

作　　者：左志晗[1,2] 郑津辉[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市细胞遗传与分子调控重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2011年第4期64-69,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津师范大学博士基金资助项目(52X0910)

摘　　要：在棒状链霉菌B71-14中扩增对克拉维酸具有正调控作用的基因ccaR,构建了ccaR的重组质粒pSET152-ccaR,通过接合转移将重组质粒pSET152-ccaR转入了S.clavuligerus B71-14中,通过pSET152-ccaR中的attP位点整个质粒插入到S.clavuligerus B71-14基因组中的attB位点,实现了S.clavuligerus B71-14基因组DNA中增加一个拷贝ccaR基因的目的,所得突变株S.clavuligerus::ccaR产酸量可达820.91mg/L,较出发菌株提高了54%.Clavulanic acid positive regulation gene ccaR was amplified in Streptomyces clavuligerus B71-14.ccaR expression plasmid pSET152-ccaR was constructed and transformed into S.clavuligerus B71-14 by conjugation.The attP site in pSET152-ccaR could be inserted into attB site of S.clavuligerus B71-14,and an extra copy of ccaR was inserted into the chromosome of S.clavuligerus B71-14.The clavulanic acid yield of the obtained mutant S.clavuligerus：： ccaR can reach 820.91 mg/L,which is 1.54 times of that with S.clavuligerus B71-14.The yield is steady within five generations.

关 键 词：克拉维酸 棒状链霉菌 ccaR[Q812] 

分 类 号：Q939.9[生物学—微生物学]