巨噬细胞移动抑制因子表达与复发性自然流产关系  

THE RELATIONSHIP BETWEEN MACROPHAGE MIGRATION INHIBITORY FACTOR EXPRESSION AND RECURRENT SPONTANEOUS ABORTION

作　　者：孙素梅[1] 刘芙蓉[1] 詹瑛[1] 叶元华[1] 张宁[1] 

机构地区：[1]青岛大学医学院附属医院产科,山东青岛266003

出　　处：《青岛大学医学院学报》2011年第5期409-412,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨巨噬细胞移动抑制因子（MIF）在早期妊娠妇女血清和妊娠组织中的表达及其与复发性自然流产（RSA）的关系。方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接（SP）方法,检测35例RSA病人、45例人工流产的正常妊娠妇女（对照组）早期妊娠绒毛和蛻膜组织中MIF的定位与蛋白表达,采用RT-PCR技术检测绒毛和蛻膜组织MIF mRNA表达水平,采用酶联免疫吸附试验（ELISA）检测血清MIF水平。结果两组孕妇绒毛滋养层细胞和蜕膜细胞中均有MIF表达,RSA组绒毛滋养层细胞胞质和蜕膜细胞胞质中MIF表达呈淡棕色,而对照组中呈深棕色。RSA组绒毛滋养层细胞胞质和蜕膜组织MIF蛋白和MIF mRNA表达均明显低于对照组,差异有显著性（t=7.089~40.610,P〈0.01）。RSA组血清MIF水平明显低于对照组,差异有显著意义（t=8.903,P〈0.01）。早期妊娠血清MIF水平与绒毛和蛻膜组织中的MIF mRNA表达水平呈正相关（r=0.873、0.657,P〈0.01）;与MIF蛋白表达水平也呈正相关（r=0.794、0.616,P〈0.01）。结论 MIF mRNA及其蛋白在RSA病人绒毛及蜕膜组织中低表达可能参与了RSA的发病,早期妊娠血清MIF水平下降对RSA的发生有一定的预测作用。Objective To investigate the expression of macrophage migration inhibitory factor（MIF） in serum and villi in early pregnancy and their association with recurrent spontaneous abortion（RSA）. Methods A total of 35 RSA patients（RSA group） and 45 normal early pregnant women undergoing artificial abortion（control group） were recruited.The expressions of MIF mRNA and MIF protein in villi and deciduas were determined by SP method;the MIF mRNA expression in villi and deciduas by reverse transcriptase-polymerase chain reaction（RT-PCR）;the serum MIF levels were detected by using enzyme-linked immunosorbent assay（ELISA）. Results MIF protein was expressed in trophoblastic cells and decidual cells of both groups,the color of MIF in RSA was pale brown,that in the control was dark brown.The expressions of MIF protein and MIF mRNA in the pregnant tissues of RSA group were significantly lower than the control group,showing a significant difference between the two groups（t=7.089-40.610,P0.01）.The serum MIF levels in RSA group were lower than that in the control group（t=8.903,P0.01）.The serum levels of MIF in early pregnancy were positively correlated with the expression of MIF mRNA（r=0.873,0.657;P0.01）,and MIF protein in villi and decidua（r=0.794,0.616;P0.01）. Conclusion Low expression of MIF mRNA and its protein in villi and decidua of RSA patients are probably involved in the pathogenesis of the disease,a decline of serum MIF in early pregnancy is of a predictive role for the occurrence of this condition.

关 键 词：流产 习惯性 巨噬细胞移动抑制因子 绒毛膜 蜕膜 

分 类 号：R714.21[医药卫生—妇产科学]