反相高效液相色谱法测定三七破壁粉粒的皂苷类含量  

Determination of Ginsenosides in Cell-broken Powder of Radix Notoginseng by RP-HPLC

作　　者：刘敏[1,2] 严萍[1,2] 詹若挺[1,2] 邓雯[2,3] 陈蔚文[1,2] 成金乐[2,3] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心,广州510006 [2]教育部省部共建中药资源科学重点实验室,广州510006 [3]中山中智药业集团有限公司,广东中山528437

出　　处：《中药新药与临床药理》2011年第6期673-676,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省粤港关键领域重点突破招标项目(20080106-2);广东省科技计划项目(粤科计字[2008]72号);中山市科技强企支撑计划项目(20092A113)

摘　　要：目的建立三七破壁粉粒的含量测定方法。方法利用Waters高效液相色谱仪测定三七破壁粉粒的三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量,流动相为乙腈-水,流速为1.0 mL.min-1,测定波长为203nm。结果三七皂苷R1在0.22～2.19μg范围内线性关系良好,平均回收率为103.12%,RSD值为1.44%;人参皂苷Rg1在0.79～7.90μg范围内线性关系良好,平均回收率为102.23%,RSD值为1.97%;人参皂苷Rb1在0.85～8.53μg范围内线性关系良好,平均回收率为96.63%,RSD值为0.99%。结论此法简便、快速,适用于三七破壁粉粒的含量测定,为进一步研究三七破壁粉粒的质量打下了基础。Objective To develop a determination method for cell-broken powder of Radix Notoginseng.Methods High performance liquid chromatography was used for the determination of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Rb1.The mobile phase consisted of acetonitrile and water,flow rate was 1.0 mL·min-1,and the wavelength was 203 nm by ultraviolet spectrophotometer.Results Standard curve regression equation of notoginsenoside R1 had a good linearity in the range of 0.22～2.19 μg,and the average recovery was 103.12 % with RSD of 1.44 %.Ginsenoside Rg1 had a good linearity in the range of 0.79～7.90 μg,and the average recovery was 102.23 % with RSD of 1.97 %.Ginsenoside Rb1 had a good linearity in the range of 0.85～8.53 μg,and the average recovery was 96.63 % with RSD of 0.99 %.Conclusion The method is simple and quick,and can be used as the determination method for cell-broken powder of Radix Notoginseng.

关 键 词：三七 破壁粉粒 反相高效液相色谱法 皂苷 

分 类 号：R284.1[医药卫生—中药学]