宁血饮对ITP模型小鼠血小板系统和免疫功能的影响  

Effects of Ningxueyin on platelet system and immune function of mice in ITP model

作　　者：杨艳梅[1] 熊德上[1] 徐酉华[2] 于洁[2] 戴碧涛[2] 

机构地区：[1]重庆医科大学中医药学院,400016 [2]重庆医科大学附属儿童医院,400014

出　　处：《重庆医学》2011年第22期2199-2201,2205,共4页Chongqing medicine

基　　金：重庆市卫生局立项课题(2005-B-42)

摘　　要：目的观察宁血饮对特发性血小板减少性紫癜(ITP)模型小鼠血小板系统及免疫功能的影响。方法建立ITP模型小鼠,并分为空白对照组,模型组,筪血小板胶囊组,宁血饮高、低剂量组,每组12只。于造模后第7天开始分组给药,连续给药8 d。检测各组血小板计数,外周血T淋巴细胞亚群、血小板相关抗体,骨髓巨核细胞计数和产板巨核细胞计数等,称脾脏质量,计算脾脏系数。结果宁血饮高剂量组血小板计数升高,骨髓巨核细胞计数减少,产板巨核细胞计数增多,血小板相关抗体水平降低,脾脏质量及脾脏系数减少,T淋巴细胞亚群失调得到改善,与模型组比较差异有统计学意义(P<0.05)。升血小板胶囊组血小板计数升高,骨髓巨核细胞计数减少,产板巨核细胞计数增多,与模型组比较差异有统计学意义(P<0.05)。宁血饮低剂量组血小板系统有所改善,但与模型组比较差异无统计学意义(P>0.05)。结论宁血饮高剂量对ITP模型小鼠血小板系统和免疫相关指标的异常有明显改善、调节作用,而升血小板胶囊仅能改善血小板系统,宁血饮低剂量则无明显影响。Objective To view the effects of Ningxueyin on platelet system and immune function of mice in idiopathic thrombocytopenic purpura（ITP） model.Methods The ITP model was established and the mice were divided into 5 groups： blank control group,model group,platelet capsule group,low-and high-dose Ningxueyin groups.Drugs were administrated in each group 7 days after the model was established.Platelet count,peripheral blood T lymphocyte subsets and platelet associated antibodies in peripheral blood platelet were tested.Megakaryocyte and giant middle plate were accounted;spleen was weighed and its coefficient was calculated.Results In high-dose Nin xue yin group,platelet count was increased,megakaryocyte count was reduced but giant middle plate count was increased.The level of platelet-associated antibodies was lower.Spleen weight and spleen coefficient were decreased.T lymphocyte subsets disorder were improved,significantly different from those of control group（P0.05）.In platelet capsule group,platelet count was increased,megakaryocyte count was reduced but giant plate count was increased,significantly different from those of control group（P0.05）.In low-dose Nin xue yin group,the platelet system was improved,but no significant difference was observed compared with the control group（P0.05）.Conclusion High-dosed Ningxueyin can obviously regulate the abnormality of platelet and immune-related indicators in ITP mice model.Platelet capsule can only regulate hematopoietic system while low-dosed Ning has no effect on them.

关 键 词：紫癜 血小板减少性 特发性 小鼠 血小板 免疫 

分 类 号：R285.5[医药卫生—中药学]