骨碎补对人牙龈成纤维细胞超微结构和碱性磷酸酶活性的影响  

Effects of rhizoma drynariae on the ultramicrostructure and the activity of alkaline phosphatase of human gingival fibroblasts

作　　者：许彦枝[1] 朱曚曚[1] 王琨[1] 

机构地区：[1]河北医科大学第四医院口腔科,石家庄050011

出　　处：《中国实用口腔科杂志》2008年第3期157-160,共4页Chinese Journal of Practical Stomatology

摘　　要：目的探讨中药骨碎补对体外培养的人牙龈成纤维(HGF)细胞超微结构、碱性磷酸酶(ALP)活性的影响。方法2005年9月至11月于河北医科大学第四医院实验中心将体外培养的HGF细胞分别与质量浓度为10、50、100、500、1000μg/mL的骨碎补共同孵育,四甲基偶氮唑盐(MTT)比色法测定ALP活性,选出ALP活性最强的骨碎补质量浓度作为最佳浓度,并作用于培养细胞。电镜下观察细胞的超微结构改变。结果在骨碎补各质量浓度范围内,实验组ALP活性均高于对照组(P均<0.05),骨碎补质量浓度为100μg/mL时ALP活性最强。与对照组比较,实验组细胞器数量增加,细胞体较大,有丰富的胞浆,胞浆内粗面内质网数量明显增多,线粒体较为发达,细胞核及核仁体积较大。结论骨碎补在一定质量浓度范围内可使ALP活性增强,细胞器数量增加,功能增强。Objective To study the effects of rhizoma drynariae on the ultramicrostructure and the activity of alkaline phosphatase(ALP)of human gingival fibroblast(HGF)cultured in vitro.Methods HGFs were inclubated with different concentrations of rhizoma drynariae respectively and ALP was measured by MTT assay to search for the best concentration.Effects of rhizoma drynariae by this best concentration on ultramicrostructure was studied by electron microscope.Results Rhizoma drynariae at selected concentrations increased ALP expression(P<0.05),especially at 100 μg/mL.At 100 μg/mL,the cell apparatus increased in number and was reinforced in function.Conclusion Proper concentrations of rhizoma drynariae may promote ALP expression of HGFs,increasing the number of cell apparatus and reinforcing its function.

关 键 词：人牙龈成纤维细胞 骨碎补 碱性磷酸酶 超微结构 

分 类 号：R285.5[医药卫生—中药学]