Establishment of Somatic Cell Clones in Thesium chinense Turcz and Its in vitro Rooting Technique  

百蕊草无性系建立与瓶外生根研究(英文)

作　　者：江芹[1] 董玲[1] 宁志怨[1] 陈静娴[1] 廖华俊[1] 

机构地区：[1]安徽省农业科学院园艺研究所,安徽合肥230031

出　　处：《Agricultural Science & Technology》2008年第5期47-49,62,共4页农业科学与技术（英文版）

基　　金：Supported by the Key Project for Supporting New Subject from Anhui Academy of Agricultural Sciences~~

摘　　要：[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.

关 键 词：Thesium chinense Turcz Clustered SHOOTS ROOTING 

分 类 号：S567.239[农业科学—中草药栽培]