系统性红斑狼疮患者CD4^+T细胞基因表达谱的研究  

Gene profiling involved in immature CD4^+ T lymphocyte from a case of systemic lupus erythematosus

作　　者：郝飞[1] 邓永键[1] 周村建[1] 游弋[1] 王继文[1] 宋志强[1] 向明明[1] 钟白玉[1] 

机构地区：[1]第三军医大学西南医院,重庆400038

出　　处：《实用皮肤病学杂志》2008年第1期16-18,5,共4页Journal of Practical Dermatology

摘　　要：目的阐明系统性红斑狼疮(SLE)CD4^+T细胞基因表达谱的变化。方法分离1例SLE患者疾病活动状态(T4-1s)与疾病无活动状态(T4-2s)下的CD4^+T细胞,应用长标签基因表达系列分析技术(LongSAGE),获得CD4^+T细胞在这两种情况下的基因表达谱数据库。选取289个具有明显表达量差异(疾病活动状态与疾病无活动状态下表达量相差4个拷贝以上)且与独特基因相匹配的基因,在PubMed上进行文献挖掘,获得相关的功能信息并进行聚类分析。结果发现基因功能的异常涉及到细胞生物学功能的多个方面,包括与CD4^+T细胞的异常发育、调节T细胞的分化与增殖、不明原因的病毒感染可能启动了自身免疫过程以及参与细胞的凋亡过程等众多的基因表达信息。结论CD4^+T细胞功能的异常有助于阐明SLE的发病机制,CD4^+T细胞的不成熟性可能是SLE致病的重要原因。Objective We attempted to characterize the genes expression of CD4^+ T lymphocytes for the pathogenesis of systemic lupus erythematosus(SLE).Methods Genomewide gene expression profiles of CD4^+ T cells,which were isolated from the disease severe activity(T4-1s) and nonactivity(T4-2s) with an SLE patient by using long serial analysis of gene expression (LongSAGE).We picked out 289 genes matching to Unigene cluster with different expression more than 4 copies between T4- 1s and T4-2s libraries and analyzed their roles from the collectedly published articles of PubMed by genes functional clustering. Results The gene functions were related to a diverse cellular process including the abnormal development of CD4^+ T cells of SLE, modulation proliferation and differentiation of T lymphocytes,initiate autoimmunity related to certain viral infections and apoptosis relating genes.Conclusion Abnormalities findings of multiple genes expression involving with a variety of CD4^+ T cells process might be meaningful to understanding the pathogenesis of SLE,and immature CD4^+ T cells might be responsible for SLE.

关 键 词：红斑狼疮 系统性 CD4^+T细胞 基因表达谱 

分 类 号：R593.241[医药卫生—内科学]