姜黄素对HaCaT细胞中成纤维细胞生长因子10影响的实验研究  

Experimental study on the effect of curcumin on fibroblast growth factor 10 in HaCaT cells

作　　者：于春水[1] 眭维耻[1] 林茂[1] 牟韵竹[1] 陈燕[1] 陈星[1] 谭升顺[2] 

机构地区：[1]川北医学院附属医院皮肤科,四川南充637000 [2]西安交通大学第二医院皮肤科

出　　处：《实用皮肤病学杂志》2008年第4期204-206,共3页Journal of Practical Dermatology

摘　　要：目的观察不同剂量姜黄素对HaCaT细胞中FGF10 mRNA转录及其蛋白翻译的影响。方法HaCaT细胞经不同剂量的姜黄素处理48h后,采用免疫荧光和原位杂交的方法检测FGF10 mRNA转录及其蛋白翻译的改变。结果与未处理组比较,处理48h后,FGF10 mRNA转录及其蛋白翻译水平随姜黄素剂量的增加而减少,两组间比较有显著性差异(P<0.05)。结论姜黄素可抑制HaCaT细胞中FGF10 mRNA转录及其蛋白翻译,并随姜黄素剂量的增加,抑制作用加强。Objective To further illuminate the effect of curcumin on FGF10 mRNA transcription and its protein translation in HaCaT cells.Methods HaCaT cells were treated with different concentration of curcumin for 48h,then the transcription of FGF10 mRNA and the translation of FGF10 protein in these cells were detected by immunofluorescence and in situ hybridization assay.Results Compared with the control groups,with the curcumin concentration increased,FGF10 mRNA transcription and its protein translation levels were decreased.Statistically significant difference was found among different groups(P<0.05).Conclusion Curcumin could have a dose-dependent inhibitory effect on FGF10 mRNA transcription and its protein translation in HaCaT cells.

关 键 词：姜黄素 HACAT细胞 成纤维细胞生长因子10 

分 类 号：R285[医药卫生—中药学]