蚯蚓冻干粉对高血脂小鼠胆固醇7α-羟化酶基因的调节作用  

Regulation of the Lyophilized Powder of Earthworm on Cholesterol 7α-Hydroxylase Gene in Hyperlipidemic Mice

作　　者：武金霞[1] 刘雪英[1] 单彪[1] 张贺迎[2] 

机构地区：[1]河北大学生命科学学院 [2]河北大学生物工程技术研究中心,河北保定071002

出　　处：《中国医药工业杂志》2011年第4期262-264,共3页Chinese Journal of Pharmaceuticals

摘　　要：以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型,灌胃给予高、中、低剂量(0.4、0.2和0.1 g/kg)蚯蚓冻干粉,每天一次,同时饲喂高脂饲料,共饲养10周。提取小鼠肝脏总RNA,应用逆转录-聚合酶链反应(RT-PCR)技术检测胆固醇7α-羟化酶(CYP7α1)的mRNA水平。结果表明,实验组小鼠的CYP7α1 mRNA相对含量显著高于高脂饲料组小鼠,提示蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平可能与上调肝CYP7α1 mRNA的表达有关。Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemic mouse model.The mice were treated with the lyophilized powder of earthworm at dosages of 0.4,0.2 and 0.1 g/kg,qd and were fed with high-level lipid feeds for 10 weeks.The total RNA of liver was extracted and the changes of liver cholesterol 7α-hydroxylase（CYP7α1） gene expression were analyzed by RT-PCR.The results indicated that the lyophilized powder of earthworm could significantly up-regulated CYP7α1 mRNA transcription.It suggested that the effect of the lyophilized powder of earthworm on reducing serum cholesterol in hyperlipidemia mice was probably involved in up-regulating CYP7α1 mRNA transcription.

关 键 词：蚯蚓冻干粉 高血脂症 胆固醇代谢 胆固醇7Α-羟化酶 

分 类 号：R96[医药卫生—药理学]