中成药中违禁添加化学止咳平喘药的HPLC-DAD法检测  

Determination of Nine Antitussive and Antiasthmatic Chemical Drugs Added Illegally in Traditional Chinese Medicines by HPLC-DAD

作　　者：刘志杰[1] 李东辉[1] 饶金华[1] 张静[1] 封衍萍 

机构地区：[1]北京市海淀区药品检验所,北京100083

出　　处：《中国医药工业杂志》2011年第4期289-291,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了HPLC-DAD法检查中药制剂违禁添加的9种化学止咳平喘药——富马酸酮替芬、硫酸特布他林、茶碱、乙羟茶碱、咖啡因、盐酸克伦特罗、愈创甘油醚、氢溴酸右美沙芬和盐酸氨溴索。采用C18色谱柱,以0.75%磷酸-甲醇为流动相,梯度洗脱,检测波长254 nm。富马酸酮替芬和愈创甘油醚在0.6～6μg、其它7种药物在0.2～2μg范围内线性关系良好,回收率为98.3%～99.6%,RSD均小于1.2%。An HPLC-DAD method was established for the determination of nine antitussive and antiasthmatic chemical drugs-ketotifen fumarate,terbutaline sulfate,theophylline,etofylline,caffeine,clenbuterol hydrochloride,guaifenesin,dextromethorphan hydrobromide and ambroxol hydrochloride,added illegally in traditional Chinese medicines.A C18 column was used with 0.75% phosphoric acid-methanol as the mobile phase by gradient elution at the detection wavelength of 254 nm.The calibration curves of ketotifen fumarate and guaifenesin were both linear in the range of 0.6-6 μg,while those of the others were linear in the range of 0.2-2 μg.The recoveries were 98.3%-99.6%,with RSDs less than 1.2%.

关 键 词：化学止咳平喘药 中药 高效液相色谱-二极管阵列检测器 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]