氧化石墨烯-硫堇及纳米金修饰玻碳电极电流型葡萄糖生物传感器的研究  

Graphene oxide-thionine and gold nanoparticles-functionalized amperometric biosensor for determination of glucose

作　　者：李岩[1] 唐点平[1] 

机构地区：[1]福州大学化学化工学院,食品安全分析与检测技术教育部重点实验室,福建福州350108

出　　处：《福州大学学报（自然科学版）》2011年第5期781-785,共5页Journal of Fuzhou University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(21075019);教育部博士点基金资助项目(2010351412003)

摘　　要：以玻碳电极为基底,在电极表面修饰一层氧化石墨烯-硫堇(GO-Th)薄膜,通过层层自组装方式,将纳米金和葡萄糖氧化酶(GOD)固定在玻碳电极表面,制得一种新型电流型葡萄糖生物传感器.在优化的实验条件下,该生物传感器对葡萄糖的线性响应范围为1.0×10-9～5.7×10-5 mol.L-1,检测下限为5.0×10-10mol.L-1.该传感器具有制备方法简单、灵敏度高、稳定性好等特点.A new amperometric biosensor for the determination of glucose was proposed by using gold nanoparticles and graphene oxide-thionine conjugates as matrices.Thionine molecules were initially adsorbed onto the surface of graphene oxide via π-π binding reaction,then the thionine-graphene conjugations were immobilized on a glassy carbon electrode,which were used for the adsorption of gold nanoparticles,and then glucose oxidase was conjugated onto the gold nanoparticles.Under optimal conditions,the biosensor exhibited a wide linear range of 1.0×10-9~5.7×10-5 mol·L-1 with a low detection limit of 5.2×10-10 mol·L-1 glucose（at S/N=3）.In addition,the stability,reproducibility and stability of the biosensor was acceptable.

关 键 词：葡萄糖 电流型生物传感器 纳米金溶胶 氧化石墨烯 硫堇 

分 类 号：O657.32[理学—分析化学]