Genetic Quality Analysis of Xiphophorus helleri RR-B Strain Using 18 Microsatellite Loci  

利用18个微卫星座位对剑尾鱼RR-B系遗传质量的分析(英文)

作　　者：刘宇飞[1,2] 白俊杰[1] 全迎春[1] 叶星[1] 黄志斌[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海200090

出　　处：《Agricultural Science & Technology》2008年第6期55-58,101,共5页农业科学与技术（英文版）

基　　金：Supported by Science and Technology Planning Project of Guangdong Province(2005B20301014);Program of National Science and Technology Platform System of China(2005DKA21103)~~

摘　　要：[Objective] The study aimed to further understand the genetic quality of Xiphophorus helleri RR-B strain and estimate the feasibility of microsatellite markers in genetic monitoring of aquatic laboratory animals.[Method] Eighteen microsatellite loci which had polymorphism in wild X. helleri were used for genetic quality analysis of RR-B strain (red eyes and red body) by PCR. [Result] All these 18 microsatellite loci displayed single allelic gene band in 30 individuals of X. helleri RR-B strain and the average homozygote rate reached 100%. [Conclusion] These loci could be used as markers in genetic monitoring of X. helleri RR-B strain, which had obtained fairly high genetic homozygosity by inbreeding for more than 20 generations. This study provided basic data for establishing the molecular genetic monitoring standard of aquatic laboratory animals.[Objective] The study aimed to further understand the genetic quality of Xiphophorus helleri RR-B strain and estimate the feasibility of microsatellite markers in genetic monitoring of aquatic laboratory animals.[Method] Eighteen microsatellite loci which had polymorphism in wild X. helleri were used for genetic quality analysis of RR-B strain (red eyes and red body) by PCR. [Result] All these 18 microsatellite loci displayed single allelic gene band in 30 individuals of X. helleri RR-B strain and the average homozygote rate reached 100%. [Conclusion] These loci could be used as markers in genetic monitoring of X. helleri RR-B strain, which had obtained fairly high genetic homozygosity by inbreeding for more than 20 generations. This study provided basic data for establishing the molecular genetic monitoring standard of aquatic laboratory animals.

关 键 词：XIPHOPHORUS helleri INBRED STRAIN MICROSATELLITE GENETIC MONITORING 

分 类 号：S917.4[农业科学—水产科学]