Optimization for Prokaryotic Expression of MCP Gene of Red-spotted Grouper Nervous Necrosis Virus  

赤点石斑鱼神经坏死病毒MCP基因原核表达条件优化(英文)

作　　者：苏友禄[1] 冯娟[1] 孙秀秀[2] 郭志勋[1] 闫云锋[1,3] 黄剑南[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]华南农业大学兽医学院,广东广州510642 [3]上海海洋大学生命科学与技术学院,上海200090

出　　处：《Agricultural Science & Technology》2008年第6期59-63,共5页农业科学与技术（英文版）

基　　金：Supported by Science and Technology Planning Project of Guangdong Province,China(2003B21502,2005B20301016)~~

摘　　要：[Objective] To optimize the prokaryotic expression of MCP gene of red-spotted grouper nervous necrosis virus. [Method] The MCP gene was amplified from red-spotted grouper nervous necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21(DE3)plysS to express proteins with induction in different media, at different pH, or at different temperatures. [Result] The expression level of recombinant bacteria reached a peak with induction under the following condition: SOB or LB medium, pH 7.0, 37 ℃ while the fusion protein was about 44.5 kD in molecular weight. [Conclusion] This study provided a basis for the development of RGNNV-MCP vaccine.[Objective] To optimize the prokaryotic expression of MCP gene of red-spotted grouper nervous necrosis virus. [Method] The MCP gene was amplified from red-spotted grouper nervous necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21(DE3)plysS to express proteins with induction in different media, at different pH, or at different temperatures. [Result] The expression level of recombinant bacteria reached a peak with induction under the following condition: SOB or LB medium, pH 7.0, 37 ℃ while the fusion protein was about 44.5 kD in molecular weight. [Conclusion] This study provided a basis for the development of RGNNV-MCP vaccine.

关 键 词：EPINEPHELUS akaara NNV MCP Expression conditions 

分 类 号：S943[农业科学—水产养殖]