Role of CaECM25 in cell morphogenesis,cell growth and virulence in Candida albicans  

作　　者：ZHANG TingTing LI WanJie LI Di WANG Yue SANG JianLi 

机构地区：[1]Key laboratory of Cell Proliferation and Regulation of Ministry of Education,Beijing 100875,China [2]Candida albicans Molecular and Cell Biology Laboratory,Institute of Molecular and Cell Biology,Proteos,61 Biopolis Drive,Sin-gapore 138673

出　　处：《Science China(Life Sciences)》2008年第4期362-372,共11页中国科学（生命科学英文版）

基　　金：the Joint Research Fund for Overseas Natural Science of China(Grant No.30228001);National Basic Research Program of China(Grant No.2007CB914401)

摘　　要：Candida albicans is the most prominent opportunistic fungal pathogen in humans. Multiple factors are associated with the virulence of C. albicans, including morphogenesis, cell wall organization and growth rate. Here, we describe the identification and functional characterization of CaECM25, a gene that has not been reported before. We constructed Caecm25?/? mutants and investigated the role of the gene in morphogenesis, cell wall organization and virulence. CaECM25 deletion resulted in defects in cell separation, a slower growth rate, reduced filamentous growth and attenuated adherence to plastic surfaces. The Caecm25?/? mutant was also significantly less virulent than wild type when tested for systemic infection in mice. Therefore, CaECM25 plays important roles in morphogenesis, cell wall organization and virulence.Candida albicans is the most prominent opportunistic fungal pathogen in humans. Multiple factors are associated with the virulence of C. albicans, including morphogenesis, cell wall organization and growth rate. Here, we describe the identification and functional characterization of CaECM25, a gene that has not been reported before. We constructed Caecm25?/? mutants and investigated the role of the gene in morphogenesis, cell wall organization and virulence. CaECM25 deletion resulted in defects in cell separation, a slower growth rate, reduced filamentous growth and attenuated adherence to plastic surfaces. The Caecm25?/? mutant was also significantly less virulent than wild type when tested for systemic infection in mice. Therefore, CaECM25 plays important roles in morphogenesis, cell wall organization and virulence.

关 键 词：Candida albicans CaECM25 gene knockout MORPHOGENESIS VIRULENCE 

分 类 号：R379[医药卫生—病原生物学]