Optimization of Culture Techniques for DH Line in Brassica napus L.  

甘蓝型油菜DH系培养技术优化(英文)

作　　者：李超[1,2] 林茂 杨斌 肖华贵 李加纳[2] 饶勇 

机构地区：[1]贵州省油料研究所,贵州贵阳550006 [2]西南大学农学与生物科技学院,重庆400716

出　　处：《Agricultural Science & Technology》2008年第4期73-77,共5页农业科学与技术（英文版）

基　　金：Supported by the Fund of Science and Technology in Guizhou Province[(2007)0281];the Fund of Guizhou Academy of Agricultural Sciences[(2007)013];the Nomarch Special Foundation for the Excellent Science and Technology Talents of Guizhou Province[(2005)40];Key Science and Technology Project in Guizhou Province[(2006)3008];Program from Science and Technology Department of Guizhou Province[(2008)-008]~~

摘　　要：[Objective] The aim of this study is to reveal the disinfectants and disinfection methods,medium components,and embryoid culture method on dissociative microspore culture.[Method] B5 as basic medium appended with different concentrations of sucrose,agar and different hormone combinations was used to optimize the culture technique for DH line in Brassica napus L.[Result] Both the 15 min disinfection of NaClO containing 5% Cl-and 10 min disinfection of 0.1% HgCl2 performed well in disinfection and subsequent embryo production;in the extraction process of dissociative microspores,B5 medium containing 2% sucrose could achieve a good embryo production effect;under dark condition microspores were firstly incubated at 32 ℃ 5-7 d,then at 25 ℃ 12-15 d,and finally transferred to 25 ℃ oscillator(60-65 r/min)for 3-7d,when the embryoid would become full ripeness;1/2MS medium appended with 1.2% agar,0.02% NAA,2.0 mg/L 6-BA,3.4 mg/L AgNO3 and 2% sucrose was helpful for embryoid differentiation and plantlet generation,presenting low degree of browning and slight vitrification.[Conclusion] The results may facilitate DH Line in rape production in large scale and high efficient transformation system.[Objective] The aim of this study is to reveal the disinfectants and disinfection methods,medium components,and embryoid culture method on dissociative microspore culture.[Method] B5 as basic medium appended with different concentrations of sucrose,agar and different hormone combinations was used to optimize the culture technique for DH line in Brassica napus L.[Result] Both the 15 min disinfection of NaClO containing 5% Cl-and 10 min disinfection of 0.1% HgCl2 performed well in disinfection and subsequent embryo production;in the extraction process of dissociative microspores,B5 medium containing 2% sucrose could achieve a good embryo production effect;under dark condition microspores were firstly incubated at 32 ℃ 5-7 d,then at 25 ℃ 12-15 d,and finally transferred to 25 ℃ oscillator(60-65 r/min)for 3-7d,when the embryoid would become full ripeness;1/2MS medium appended with 1.2% agar,0.02% NAA,2.0 mg/L 6-BA,3.4 mg/L AgNO3 and 2% sucrose was helpful for embryoid differentiation and plantlet generation,presenting low degree of browning and slight vitrification.[Conclusion] The results may facilitate DH Line in rape production in large scale and high efficient transformation system.

关 键 词：BRASSICA NAPUS L. DH LINE MEDIUM EMBRYOID 

分 类 号：S565.4[农业科学—作物学]