Providencia rettgeri strain HNR菌株羟胺氧化酶超声波法提取的研究  

Study on Hydroxylamine Oxidase Extraction of Providencia rettgeri strain HNR with Ultrasonic Cell-break Method

作　　者：黄珏[1] 何义亮[1] 赵彬[1] Shauna Taylor 张文英[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《生物技术通报》2008年第S1期384-388,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(No.20676078)

摘　　要：为了更有效和便捷的提取菌株脱氮过程中的关键酶,本文利用超声波破碎法对异养脱氮菌Providencia rettgeri strain HNR进行破碎,通过单因素实验及正交试验,确定出最佳破碎条件,便于后续试验。实验结果表明:最佳超声破碎条件为菌液OD_(600)为1.996,工作次数为70次,工作/间歇为5/5s功率为400W的组合为最佳工作条件。在此工作条件下破碎后得到的酶粗提液对羟胺有降解,得到羟胺氧化酶。In order to obtain the key enzyme involved in the nitrogen removal process of Providencia rettgeri strain HNR effectively and conveniently,the ultrasonic cell-disruption technique is used.Optimum conditions were chosen based on single-factor and orthogonal experimental results.The results showed that optimum conditions for cell-breakage of Providencia rettgeri strain HNR via ultrasonication were:cell OD_(600)1.996,work times 70,work/interval time 5s/5s and ultrasonic output power 400W.The hydroxylamine oxidase was successfully extracted using the ultrasonic cell-disruption process under the optimum condition identified.This was confirmed by a positive reduction in hydroxyl amine concentrations.

关 键 词：超声波法 细胞破碎 PROVIDENCIA rettgeri STRAIN HNR 

分 类 号：Q814.1[生物学—生物工程]