极大螺旋藻藻胆蛋白提纯工艺初试  

Pilot Study on Extraction and Purification of Phycobiliproteins in Spirulina maxima

作　　者：蔡春尔[1] 柳俊秀[1] 周铭[1] 李晨[1] 马召腾[1] 何培民[1] 

机构地区：[1]上海海洋大学生命学院中心实验室,上海200090

出　　处：《生物技术通报》2008年第S1期451-455,共5页Biotechnology Bulletin

基　　金：上海高校选拔培养优秀青年教师科研专项基金(科07-13);国家863计划项目(2006AA10Z323;2007AA09Z406);上海市科委"登山行动计划"项目(06dz12015);上海市教委重点项目(07ZZ135);上海市重点学科建设项目(Y1101)的资助

摘　　要：目的:螺旋藻富含藻胆蛋白,其附加值高,本实验对螺旋藻藻胆蛋白提取工艺做初步探索;方法:比较了组织捣碎法,冻融法,超声波法释放螺旋藻藻胆蛋白的效果,并用硫酸铵盐析,羟基磷灰石层析进一步提纯,对结果作了吸收光谱和SDS电泳鉴定;结果:显示超声波法破碎效果好,两次羟基磷灰石层析所得蛋白纯度较高;结论:表明本实验流程相对以往工艺经济,省力,省时,对螺旋藻藻胆蛋白的规模提取有一定实用价值。Objective:This paper presents pilot study on extraction and purification of costliness phycobiliproteins abound in Spirulina maxima.Methods:tissue-trituration method,freezing/unfreezing method and ultrasonic method were applied and compared to prepare crude extracts.Further purification was performed by means of ammonium sulphate precipitation and hydroxylapatite(HA)column chromatogram.Purified protein were examined through absorption spectrum and SDS-PAGE.Results:The results showed that ultrasonic method was the optimal method for extracting phycobiliproteins, and twice hydroxylapatite(HA)column chromatograms have to be performed to obtain fine product.Conclusion:considering cost of economy,manpower and time,this technology is useful in large-scale isolation and purification of phycobiliproteins in Spirulina maxima.

关 键 词：极大螺旋藻 藻胆蛋白 提取 纯化 纯度 产率 

分 类 号：Q946[生物学—植物学]