支气管哮喘诱导痰中嗜酸粒细胞凋亡及其临床意义  

The EOSinophil apoptosis in the sputum of induced patients with bronchial asthma and its clinical significance

作　　者：刘洋[1] 陈丽萍[1] 王昌亮[2] 张国华[2] 

机构地区：[1]沈阳医学院沈洲医院呼吸内科,沈阳110002 [2]中国医科大学法医学院法医病理室,沈阳110001

出　　处：《中国实用内科杂志》2008年第S2期129-131,共3页Chinese Journal of Practical Internal Medicine

基　　金：沈阳市科委自然科学基金(199943014)

摘　　要：目的观察支气管哮喘患者诱导痰中嗜酸粒细胞(EOS)凋亡的超微结构变化,并探讨其临床意义。方法对2000年8月至2007年12月沈阳医学院沈洲医院门诊及住院的90例支气管哮喘患者,用3%高渗盐水超声雾化吸入进行痰液诱导,观察哮喘患者治疗前后诱导痰中EOS凋亡的超微结构变化。结果 (1)治疗前哮喘患者茶碱组、激素组、联合用药组的FEV1分别为(1.91±0.23)L、(1.96±0.27)L、(2.02±0.32)L,与治疗后的3组FEV1(2.2±0.22)L、(2.29±0.24)L、(2.51±0.18)L相比较,差异有统计学意义(P<0.05),且联合用药组较其他两组差异有统计学意义(P<0.05)。(2)治疗前哮喘患者茶碱组、激素组、联合用药组的FEV1%分别为(73.99±2.17)%、(74.65±1.82)%、(73.63±2.63)%,与治疗后的3组FEV1%(80.16±2.85)%、(80.90±1.70)%、(85.44±2.51)%相比较,差异有统计学意义(P<0.05),且联合用药组较其他两组差异有统计学意义(P<0.05)。(3)治疗后哮喘患者茶碱组、激素组、联合用药组的总IgE较治疗前,差异有统计学意义(P<0.05),且联合用药组与其他两组比较,有统计学意义(P<0.05)。(4)在透射电镜下观察哮喘患者治疗后诱导痰中的EOS,有典型的凋亡特征。结论EOS凋亡是支气管哮喘的一个显著病理特征;氨茶碱、糖皮质激素都对EOS的凋亡有促进作用,联合用药对患者诱导痰中EOS凋亡有促进作用,并且EOS的凋亡特征比单独用药明显。Objective To observe the ultrastructure change of the EOSinophil(EOS) apoptosis in the sputum of induced patients with bronchial asthma and investigate its clinical significance.Methods Using 3%hypertonic saline ultrasonic atomizing inhalation to induce sputum in the 90 patients with bronchial asthma in shenyang hospital of shenyang medical college,From August 2000 to December 2007,and observing the ultrastructure change of the EOSinophil apoptosis in the sputum of induced patients with bronchial asthma before and after treatment.Results Before treatment,the FEV1 oftreatment group with aminophyline,with glucocorticoid and combined treatment is(1.91±0.23) L、(1.96±0.27) L、(2.02±0.32) L ampare with results,FEVl(2.2±0.22) L、(2.29±0.24) L、(2.51±0.18) L after treatment is significant different(P<0.05) ,and the result is much more significant different between the combined group and other two groups(P<0.05) .Before treatment,the FEV1% of treatment group with aminophyline,with glucocorticoid and combined treatment group is(73.99±2.17) %、(74.65±1.82) %、(73.63±2.63) %,compare with the result FEV1%(80.16±2.85) %、(80.90±1.70) %、(85.44±2.51) %after treatment is significant different(P<0.05) ,and the result is significant different between the combined group and other two groups.After treatment,the IgE of asthma patients in treatment group with aminophyline,with glueoeorticoid and combined treatment group with are different and the result is significant different between the combined group and other two groups.The EOS has the typical apoptosis characters by observation with transmission electron microscope.Conclusion The EOS apoptosis is one of the significant pathological characters of bronchial asthma.Aminophyline and glucocorticoid both can improve the EOS apoptosis.The combined treatment can significant improre the action of apoptosis in EOS promotive action on apoptosis of EOS.

关 键 词：哮喘 诱导痰 嗜酸粒细胞 凋亡 

分 类 号：R562.25[医药卫生—呼吸系统]