甘油糖脂的UPLC-Q-TOF-MS研究  

Analysis of Glycolipid Compound by UPLC-Q-TOF-MS Method

作　　者：陈德莹[1] 徐继林[1] 严小军[1] 李海英[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《分析测试学报》2008年第S1期55-58,共4页Journal of Instrumental Analysis

基　　金：教育部高等学校科技创新工程重大项目培育资金项目(705028);浙江省自然科学基金资助项目(Y506131)

摘　　要：The qualitative and quantitative research on glycolipids compounds was analyzed by means of the flight time ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-Q-TOF-MS) technology.The mass spectrometry was performed in reflective time-of-flight using electron spraying ionization.The components in monoglycosyldiglycerides(MGDG) and diglycosyldiglycerides(DGDG) were determined in positive ion mode,and the sulfoquinovosyl diacylglycerol(SQDG) were analyzed in negative ion mode.The results showed that each component in golycolipids can be separated effectively with gradient elution in the BEH C18 column.The proportion of each components accounts for total glycolipids can be determined by using degree elution.The qualitative and quantitative research on glycolipids compounds was analyzed by means of the flight time ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-Q-TOF-MS) technology.The mass spectrometry was performed in reflective time-of-flight using electron spraying ionization.The components in monoglycosyldiglycerides(MGDG) and diglycosyldiglycerides(DGDG) were determined in positive ion mode,and the sulfoquinovosyl diacylglycerol(SQDG) were analyzed in negative ion mode.The results showed that each component in golycolipids can be separated effectively with gradient elution in the BEH C18 column.The proportion of each components accounts for total glycolipids can be determined by using degree elution.

关 键 词：UPLC-Q-TOF-MS MGDG DGDG SQDG 

分 类 号：Q946[生物学—植物学]