芥蓝游离小孢子培养成胚及油菜素内脂的作用  

Eembryogenesis and effect of brassinosteroid in isolated microspore culture of Brassica alboglabra Bailey

作　　者：李智军[1] 李春艳[1] 张衍荣[1] 龙卫平[1] 郑锦荣[1] 韩福光[1] 

机构地区：[1]广东省农科集团良种苗木中心,广东广州510640

出　　处：《广东农业科学》2008年第S1期108-111,共4页Guangdong Agricultural Sciences

基　　金：广州市应用基础研究项目(2006J1-C0191)

摘　　要：芥蓝游离小孢子经32℃热激48 h后部分小孢子体积膨大3～4倍,在25℃下继续培养2 d后,可见膨大小孢子进行第1次分裂形成2细胞,4 d后分裂形成4细胞。培养14 d后,只有少部分小孢子可发育成肉眼可见的胚状体,而未膨大或异常膨大的小孢子均不能发育成胚状体。用2个基因型比较了在NLN-13培养基中添加油菜素内脂(BR)对小孢子胚产量的影响,首次发现BR对芥蓝小孢子成胚具有显著的促进作用,其有效浓度范围因基因型有所差异,翠宝的有效浓度为0.005～0.04 mg/L,圆叶则为0.005～0.01 mg/L,两基因型均以浓度0.01 mg/L的效果最佳。In isolated microspore culture of Brassica alboglabra Bailey,some microspores enlarged 3～4 times in size after 48-hour heat shock at 32℃,and their first cell divisions appeared at the second day after transferred to cultivation at 25℃in darkness,and continued to divide to form fourcells at the fourth day.Through two weeks of cultivation,only a few microspores could keep continuous development to form microspore-derived embryos,whereas no embryos developed from non-enlarged microspores or abnormally enlarged ones.Using two genotypes,Cuibao and Yuanye,the embryo yields of isolated microspores were compared in NLN-13 media supplemented with different concentrations of brassionosteride(BR).It was first found that BR had a significant promotion effect on the microspore embryogenesis of Brassica alboglabra Bailey,and the valid concentration differed with genotypes,ranging from 0.05 to 0.04 mg/L for Cuibao and from 0.005 to 0.01 mg/L for Yuanye.The best effect was at 0.01 mg/L for both genotypes

关 键 词：芥蓝 小孢子 成胚 油菜素内脂 

分 类 号：S635.9[农业科学—蔬菜学]