石墨炉原子吸收分光光度法测定大鼠血浆和尿液中的赛特铂  

The determination of satraplatin in rat plasma and urine by graphite furnace atomic absorption spectrometry

作　　者：颜冬梅[1] 马张英[2] 屠凌岚[1] 彭小英[1] 李文钧[1] 

机构地区：[1]浙江省医学科学院,杭州310013 [2]浙江大学医学院附属邵逸夫医院,杭州310016

出　　处：《中国现代应用药学》2006年第S2期731-733,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立大鼠血浆和尿液中赛特铂含量测定的无火焰石墨炉原子吸收分光光度法。方法采用岛津AA-670原子吸收分光光度仪及GFA-4A石墨炉,上海电光KY-1铂空心阴极灯,检测波长265.9 nm,测定大鼠血浆和尿液中的赛特铂含量。血尿样品用Triton X-100稀释,进样50μL。结果在浓度范围0.25 mg.L-1~10.0 mg.L-1内,样品峰面积与浓度呈良好线性关系,相关系数r2分别为血浆0.9952,尿液0.9878;加样回收率为血浆89%~112%,尿液82%~100%。结论方法简便可靠,适于生物样本中的赛特铂含量测定和动物药动学研究。OBJECTIVE To develop a rapid,accurate and sensitive method for the measurements of satraplatin in biological fluids of rats.METHODS Satraplatin in plasma and urine was analyzed by flameless atomic absorption spectrometry on an instrument Shimadzu AA-670 atomic absorption spectrophotometer equipped with a Shimadzu GFA-4A graphite furnace,and an ASC-60 autosampler.The detection wavelength was set at 265.9 nm.Plasma and urine samples were diluted with Triton X-100 before assays,fifty microliters of solution after preparation was injected to the system. RESULTS The linear ranges of satraplatin in plasma or urine were 0.25mg·L^(-1)~10.0mg·L^(-1).The correlation coefficient r^(2) was 0.9952 and 0.9878,and the regression equation were A=(0.2118)C-0.0548 and A=0.1556C-0.0042 for plasma and urine,respectively.The average recoveries were 89%~112% in plasma and 82%~100% in urine,respectively.The RSD values of interday and intra-day assays were lower than 8.9% and 6.4% respectably.CONCLUSION This method is suitable for the preclinical pharmacokinetic studies of satraplatin in rats.

关 键 词：赛特铂 大鼠 血浆 尿 原子吸收分光光度法 铂抗癌药 

分 类 号：R96[医药卫生—药理学]