复方利巴韦林滴鼻剂中主药的含量测定  

Determination method of basic remedy in Compound Ribavirin nose drops

作　　者：王兴华[1] 陈圣洁[1] 尹志萍[1] 郑清芬[1] 

机构地区：[1]河北省儿童医院药剂科,石家庄050031

出　　处：《中国现代应用药学》2006年第S2期786-787,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立复方利巴韦林滴鼻剂中主药的含量测定方法。方法以ZORBAX SB-CN(250mm×4.6mm,5μm)色谱柱,乙腈-水-磷酸(38∶62∶0.1)为流动相测定利巴韦林和盐酸苯海拉明的含量。检测波长为224nm,流速为1.0mL.m in-1。结果利巴韦林在0.1~1.0 mg.mL-1浓度范围有良好的线性关系,r=0.9990;平均回收率为100.3%,RSD=0.62%(n=9);盐酸苯海拉明回收率为100.8%,RSD=0.54%(n=9)。结论含量测定方法简单、准确和有效。OBJECTIVE To establish a method for the determination of basic remedy in Compound Ribavirin nose drops.METHODS ZORBAX SB-CN(250mm×4.6mm,5μm) column was used to determine the content of Ribavirin and Diphenhydramine Hydrochloride,and the mobile phase of acetonitrile-water-phosphoric acid(38∶62∶0.1) was pumped at 1.0mL·min^(-1) through the column.The detector was set at 224nm. RESULTS A good linear relationship were obtained between the peak area and the concentration of Ribavirin from 0.1~1.0mg·mL^(-1)(r=0.9990),and the average recovery was 100.3%,the RSD was 0.62%(n=9);the average recovery of Diphenhydramine Hydrochloride was 100.8%,the RSD was 0.54%(n=9).CONCLUSION The method for determination is simple,accurate,and relable.

关 键 词：利巴韦林 盐酸苯海拉明 含量测定 

分 类 号：R927[医药卫生—药学]