系数倍率分光光度法测定氯霉素片的含量  

Determination of Chloramphenicol in Tablets by K-ratio Spectrophotometry

作　　者：梁伟 

机构地区：[1]浙江省宁波市天衡制药有限公司,浙江宁波315201

出　　处：《中国现代应用药学》2006年第S2期799-800,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立氯霉素片的含量测定方法。方法系数倍率分光光度法测定氯霉素片的含量,以278nm为测定波长,350nm为参比波长。结果氯霉素标准品在5μg/mL^40μg/mL的范围内与ΔA呈良好的线性关系(r=1.0000),平均回收率(n=8)为99.70%,RSD=0.39%,与药典法测定结果无显著差异。结论本法操作简便、省时,结果准确可靠。OBJECTIVE To establish the determination of chloramphenicol in tablets.METHODS The determination of chloramphenicol was carried out by Kratio spectrophotometry with detecting wavelength 278 nm and refernce wavelength 350nm. RESULTS The linear range of chloramphenicol was 5μg/mL^40μg/mL(r=1.0000),The average recovery(n= 8) was 99.70%,RSD was 0.39%,The result was identieal to that assayed by the method introduced in pharmacopoeia.CONCLUSION This method is simple,the analytical results were reliable and accurate.

关 键 词：氯霉素片 含量测定 系数倍率分光光度法 

分 类 号：R927[医药卫生—药学]