ICU患者铜绿假单胞菌获得性金属β-内酰胺酶基因的研究及其耐药性分析  

The Analyses of Resistance of Pseudomonas Aeruginosa and The Study of The Acquired Metallo-β-lactamases Gene on ICU Patients

作　　者：胡锋兰[1] 黄震[2] 

机构地区：[1]深圳市南山区人民医院检验科 [2]深圳市龙岗中心医院,广东深圳518052

出　　处：《九江学院学报（自然科学版）》2011年第2期62-65,共4页Journal of Jiujiang University：Natural Science Edition

摘　　要：目的:了解我院ICU患者铜绿假单胞菌的耐药性,为临床合理使用抗生素提供依据和探讨该耐药菌的防治方法。方法:临床分离的106株铜绿假单胞菌用法国梅里埃公司的VITEK-2全自动微生物分析系统鉴定,纸片扩散法进行抗生素敏感试验,采用纸片协同法检测金属酶表型,PCR法扩增金属酶基因。结果:2007～2009年共分离出106株铜绿假单胞菌,对头孢他啶、亚胺培南、哌拉西林、氨曲南、头孢哌酮、头孢吡肟、左氧氟沙星的耐药率分别为50.9%、39.6%、79.2%、56.6%、57.5%、41.5%、25.5%。结论:携带AmpC金属酶基因是本院ICU患者铜绿假单胞菌耐碳青霉烯类等多种药物的主要原因。OBJECTIVE：Understand the ICU patients with Pseudomonas aeruginosa resistant cells for clinical use of antibiotics and provide for prevention and treatment of drug-resistant methods.METHOD：Pseudomonas aeruginosa were identified by VITEK-2 system,antimicrobial sensitivity tests were performed by K-B method and metallo-β-lactamases-producing bacteria was screened by the double disk synergy test.The metallo-β-lactamases-encoding genes were amplified by PCR.The PCR products electrophoresis were performed to sure the genotypes. RESULTS：106 strains of Pseudomonas aeruginosa were isolated.The resistant rates of antibiotics ceftazidime,imipenem,piperacillin,aztreonam,cefoperazone,cefepime,levofloxacin respectively were 50.9%,39.6%,79.2%,56.6%,57.5%,41.5% and 25.5%. CONCLUSION：The rnain reason why Pseudomonas aeruginosa isolated in our hospital shows resistant to carbanpenems and other kinds of antibiotics was AmpC gene.

关 键 词：ICU 铜绿假单胞菌 AMPC基因 耐药性 

分 类 号：R446.5[医药卫生—诊断学]