白黎芦醇对胶原合成及相关基因表达的影响  

Effect of Resveratraol on Collagen Synthesis and Gene Expression

作　　者：朱倩[1] 夏洪生 张辛[1] 陈力莹[1] 张力勇[1] 池晓霞[1] 

机构地区：[1]深圳市第二人民医院,广东518031 [2]深圳市中西医结合研究所

出　　处：《中国病毒病杂志》2005年第3期14-16,共3页Chinese Journal of Viral Diseases

基　　金：深圳市科技局立项课题(编号:200405104)

摘　　要：目的探讨白黎芦醇对人皮肤瘢痕组织成纤维细胞增殖及Ⅰ、Ⅲ型胶原合成及降解影响的机理。方法用MTT法检测及原位杂交技术,对增生性瘢痕用白黎芦醇作用不同时间后,成纤维细胞的增殖及Ⅰ、Ⅲ型前胶原mRNA的原位表达进行了研究。结果(1)白黎芦醇对人皮肤瘢痕组织成纤维细胞的抑制率随浓度的增加和作用时间的延长而增强,最大抑制率可达到93.69%。(2)Ⅰ、Ⅲ型前胶原mRNA 3 d已被明显抑制(P<0.01),7 d后表达强度进一步下降,白黎芦醇对Ⅰ型前胶原mRNA的抑制作用要比Ⅲ型前胶原强。结论(1)白黎芦醇对人皮肤瘢痕组织成纤维细胞增殖有抑制作用,且呈剂量时间依赖性。(2)白黎芦醇对Ⅰ、Ⅲ型前胶原mRNA有明显抑制作用,对Ⅰ型前胶原mRNA的抑制作用要比Ⅲ型前胶原强。Objective To study the effects of Resveratraol on viability of fibroblasts and type Ⅰand Ⅲ collagen syntheses and degradation in hypertrophic scars.Methods (1)Various concentrations of Resveratroal were added into the medium and co-incubated with FB for different time period.MTT assay was employed to detect proliferation of fibroblast;(2)With Resveratraol was performed on hypertrophic scars.On the 3^(rd) day and 7^(th) day,type Ⅰ and Ⅲ procollagen mRNA expression were studied by means of immunohistochemistry and molecular biology.Results(1)The resveratraol in various concentrations inhibited proliferation of fibroblast;(2)On the 3^(rd) day after with Resveratraol,both type Ⅰ and Ⅲ procollagen mRNA expressions were significantly inhibited and on the 7^(th) day both procollagen mRNA expressions decreased further.With Resveratraol may inhibit type Ⅰ procollagen mRNA expression more than type Ⅲ.Conclusions Resveratraol may inhibit viability of fibroblasts and type Ⅰ and Ⅲ procollagen mRNA expression.

关 键 词：白黎芦醇 瘢痕 成纤维细胞 Ⅰ、Ⅲ型前胶原基因 

分 类 号：R285.5[医药卫生—中药学]