H9N2亚型禽流感病毒细胞适应株细胞致病性和HA、NA基因序列分析  

Pathogenicity and HA,NA Genes Sequences Analysis of Avian Influenza Virus H9N2 Subtype Adapted in Mammalian Cell Line

作　　者：牛学锋[1] 罗琴芳[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《武汉大学学报（理学版）》2005年第S2期245-248,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30270987;30440010);广东省自然科学基金资助项目(020996)

摘　　要：对分离自中国南方的H9N2亚型禽流感病毒A/Chicken/Guangxi/KMⅢ/99(H9N2)进行了MDCK细胞的连续传代和NA基因序列的初步分析.研究发现,病毒接种细胞36 h左右出现细胞病变(CPE),细胞肿胀变圆,聚集,脱落.流感病毒经MDCK细胞连续传代19代后,HA效价与亲代病毒相当.将传代获得的第19代病毒进行基因克隆,与亲代病毒进行序列比较,发现经传代后其HA1羧基端的分子特征是:R-S-S-R,仍属于非低致病力毒株.The avian influenza virus was adapted to MDCK cell line by continuous passage,and the HA,NA gene sequences were analyzed.The study demonstrated that typical cytopathic effect(CPE) were seen,the HA titer was 9 log2,equal to the parental virus.The HA and NA gene showed 98%-99% nucleotide sequence homology between the variant and the parental isolate.Genetic analysis showed that the virus had the same amino acid near the HA connecting peptide: R-S-S-R.

关 键 词：禽流感病毒 MDCK细胞 HA基因 NA基因 

分 类 号：S852.65[农业科学—基础兽医学]