基于结冷胶培养基的乌梁素海水体中可培养细菌的多样性研究  

CULTIVABLE BACTERIA DIVERSITY OF WULIANGSUHAI LAKE BY USING GELLAN GUM MEDIUM

作　　者：金一荻[1] 冯福应[1] 赵宇龙[1] 张晓军[1] 

机构地区：[1]内蒙古农业大学生命科学学院应用与环境微生物实验研究所,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2011年第2期160-165,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目(3760004)

摘　　要：分别采用琼脂和结冷胶作为培养基的固体支撑物,用培养和分子相结合的方法研究乌梁素海水体中可培养细菌的多样性。对比发现在以结冷胶作为固体支撑物的培养基上生长的细菌总数约为传统琼脂培养基的3倍,此外菌落的形态、颜色等,也有很明显的不同。在结冷胶培养基上共分离得到41株纯培养物,测定其16S rDNA部分序列并据此进行分析,表明这些菌株分别属于β-Proteobacteria(48.8%)、Actinobacteria(19.5%)、γ-Proteobacteria(9.8%)、α-Proteobacteria(7.3%)、Deinococcus-Thermus(7.3%)以及Firmicutes(7.3%)。其中,与嗜氢菌属(Hydrogenophaga)发育系统关系密切的细菌为优势属。Cultivable bacteria diversity of Wuliangsuhai Lake was comparatively;studied by using traditional nutrient medium（Agar） and gellan gum medium.Results showed that the amount of the bacteria cultured on gellan gum medium was about 3 times higher of those on Agar.The 41 clones were isolated from gellan gum medium.The similarity of 16S rRNA gene sequences of the isolates was analyzed.The results indicated that on gellan gum medium the bacterial groups were β-Proteobacteria（48.8%）,Actinobacteria（19.5%）,γ-Proteobacteria（9.8%）,α-Proteobacteria（7.3%）,Deinococcus-Thermus（7.3%） and Firmicutes（7.3%）.Hydrogenophaga-related strains were predominant bacteria.

关 键 词：乌梁素海 结冷胶培养基 可培养细菌 细菌多样性 

分 类 号：Q938.8[生物学—微生物学]