斯氏狸殖吸虫感染大鼠致肝纤维化形成过程中IL-4和IFN-y表达及意义  

Expression and significance of IL-4, IFN-y in liver fibrosis of rats infected with Pagumogonimus skrjabini

作　　者：王文林[1] 陈鹏[2] 李芳[1] 王殿华[3] 

机构地区：[1]昆明医学院寄生虫学教研室 [2]河南郑州市第一人民医院 [3]昆明医学院天然药物重点实验室

出　　处：《热带病与寄生虫学》2010年第3期131-134,共4页Journal of Tropical Diseases and Parasitology

基　　金：云南省教育厅科学研究基金（No.0111437,No.06Y044C）资助项目

摘　　要：目的探讨斯氏狸殖吸虫感染大鼠致肝纤维化形成过程中细胞因子IL-4和IFN-吖表达及意义，为阐明Th1／Th2在斯氏狸殖吸虫感染大鼠肝纤维化形成及调节作用提供实验依据。方法SD大鼠36只，随机分为正常对照组（n=6）、感染1周组、感染2周组、感染4周组、感染8周组、感染12周组和感染16周组。每鼠腹腔注射斯氏狸殖吸虫囊蚴15个。按感染时间处死各组大鼠，观测各项指标。HE染色和VG染色观察肝组织纤维化病理形态学变化。免疫组织化学法测定IL-4、IFN-y在大鼠肝纤维化形成过程中的表达动态变化。结果HE染色和VG染色肝组织病理形态学结果显示：随着感染时间的延长，胶原纤维面积比值（S／T）和肝纤维化评级在逐渐提高，肝组织纤维化的程度逐渐加重。免疫组织化学显示：IFN-y的表达阳性细胞百分率在1周至8周在逐渐增加，到第8周末时达到最大，在12周到16周又逐渐减少：而IL-4表达阳性细胞百分率在1周至16周在逐渐增加．到第12周末IL-4表达开始增加迅速。结论斯氏狸殖吸虫感染大鼠可引起肝脏纤维化病变，IL-4和IFN-y在斯氏狸殖吸虫感染大鼠致肝纤维化形成过程中表达增加，其纤维化病变程度与感染时间、IL-4和IFN～1的表达增加有关。提示Th1细胞分泌的细胞因子IFN-y和Th2细胞分泌的细胞因子IL-4在斯氏狸殖吸虫感染大鼠致肝纤维化的自发性免疫调节中起作用。Objective To explore the expression and significance of interleukin-4 and interferon-y in the formation of liver fibrosis of rats infected with Pagumogonimus skrjabini, and provide experimental evidence on the role of Th1/Th2 for the formation and regulation of liver fibrosis of rats infected with Pagumogonimus skrjabini. Methods 36 rats were randomly divided into two groups, the control group and the infection group. The Infection group were divided into 6 groups according to infection time： group A （1 week after infection）, group B （2 weeks after infection）, group C （4 weeks after infection）, group D （8 weeks after infection）, group E （12 weeks after infection）, F group （16 weeks after infection）. Each rat was infected with 15 Pagumogonimus skrjabini metacercarias by the way of peritoneal injection. The rats were sacrificed and observed indicators in corresponding time after infection. The pathomorphological changes of liver fibrosis were observed by the HE staining and VG staining. The immunohistochemical technique was used to measure the dynamic changes of expression of interleukin-4, interferon-y in the formation of liver fibrosis in rats infected with Pagumogonimus skrjobini. Results The results of pathomorphology showed that the ratio of collagen fiber area （S/T） and the liver fibrosis rating increased and the degree of liver fibrosis gradually deepened gradually with the extension of infection time. The results of immunohistochemistry showed that the percentage of positive cells of INF-7 expression increased gradually at lst-Sth week after infection and reached the peak at the end of 8th week, then decreased gradually at 12th-16th week. The percentage of positive cells of IL-4 expression increased gradually at lst-16th week after infection and rose rapidly at the end of 12th week. Conclusion The study suggested that Pagumogonimus skrjabini infection in rats could cause liver fibrosis, the expression of IL-4 and IFN-7 increased in the formation of liver fibrosis of rats infect

关 键 词：IL-4 IFN-y 斯氏狸殖吸虫 肝脏纤维化 免疫组化 

分 类 号：R532.2[医药卫生—内科学]