Transformation of Streptococcus zooepidemicus with Genes Responsible for Polyhydroxybutyrate Synthesis  

作　　者：吴小明 高海军 田格 陈国强 

出　　处：《Tsinghua Science and Technology》2002年第4期387-392,共6页清华大学学报（自然科学版（英文版）

摘　　要：A procedure for transformation of intact Streptococcus zooepidemicus cells by electroporation was developed through a systematic examination of the effects of various parameters, including growth conditions, electric field strengths used for electroporation, and concentrations of plasmid used for transformation. Efficiencies higher than 10 4 cfu/μg (cfu, clone forming unit) plasmid DNA were obtained for Streptococcus zooepidemicus H2004 cells. Results demonstrate that the broad host range plasmid pDL276 can be replicated in Streptococcus zooepidemicus H2004 and foreign genes responsible for polyhydroxybutyrate (PHB) synthesis inserted into the pDL276 can be successfully expressed in the transformant, in which PHB is detected using the Fourier transform infrared spectroscopy (FT IR) method.A procedure for transformation of intact Streptococcus zooepidemicus cells by electroporation was developed through a systematic examination of the effects of various parameters, including growth conditions, electric field strengths used for electroporation, and concentrations of plasmid used for transformation. Efficiencies higher than 10 4 cfu/μg (cfu, clone forming unit) plasmid DNA were obtained for Streptococcus zooepidemicus H2004 cells. Results demonstrate that the broad host range plasmid pDL276 can be replicated in Streptococcus zooepidemicus H2004 and foreign genes responsible for polyhydroxybutyrate (PHB) synthesis inserted into the pDL276 can be successfully expressed in the transformant, in which PHB is detected using the Fourier transform infrared spectroscopy (FT IR) method.

关 键 词：ELECTROPORATION STREPTOCOCCUS polyhydroxybutyrate(PHB) Fourier transform  infrared spectroscopy (FT  IR) 

分 类 号：Q933[生物学—微生物学]