Multilayers Assembly of DNA Probe for Biosensor  

作　　者：谢文章 路英杰 隋森芳 

出　　处：《Tsinghua Science and Technology》2002年第4期430-434,共5页清华大学学报（自然科学版（英文版）

摘　　要：Surface plasmon resonance (SPR) was a sensitive method to study molecular interactions. Based on the specific binding, this paper presented the molecular assembly of protein nucleic acid multilayers on the surface of a gold film. The first layer was a biotin lipid (B DMPE/DMPE) containing a monolayer prepared using the Langmuir Blodgett (LB) technique. The second and third layers were avidin and DNA labeled biotin, respectively. The fourth layer was anti DNA antibody extracted from the serum of patients with systemic lupus erythematosus (SLE). These interactions provide stability in the multilayer films of the complexes. The multilayer formation process was detected by SPR spectroscopy. The results show that the chip based sensor system can be used for functional characterization of protein protein and protein DNA interactions. Surface plasmon resonance (SPR) was a sensitive method to study molecular interactions. Based on the specific binding, this paper presented the molecular assembly of protein nucleic acid multilayers on the surface of a gold film. The first layer was a biotin lipid (B DMPE/DMPE) containing a monolayer prepared using the Langmuir Blodgett (LB) technique. The second and third layers were avidin and DNA labeled biotin, respectively. The fourth layer was anti DNA antibody extracted from the serum of patients with systemic lupus erythematosus (SLE). These interactions provide stability in the multilayer films of the complexes. The multilayer formation process was detected by SPR spectroscopy. The results show that the chip based sensor system can be used for functional characterization of protein protein and protein DNA interactions.

关 键 词：surface plasmon resonance (SPR) Langmuir  Blodgett (LB) film molecular recognition avidin  biotin systemic lupus erythematosus (SLE) 

分 类 号：Q503[生物学—生物化学]