Sodium Acetate Stimulates PHB Biosynthesis in Synechocystis sp. PCC 6803  

作　　者：吴桂芳 鲍恬 沈忠耀 吴庆余 

出　　处：《Tsinghua Science and Technology》2002年第4期435-438,共4页清华大学学报（自然科学版（英文版）

基　　金：Supported by the National Natural Science F oundation of China (No.2 0 0 760 2 4)

摘　　要：Synechocystis sp. PCC 6803 cell growth and poly β hydroxybutyrate (PHB) biosynthesis were studied in the presence of sodium acetate (NaAc). For nitrogen sufficient conditions, 15 mmol/L NaAc improved the PHB content up to 9.9% (w/w) while for nitrogen starved conditions, the PHB content was up to 15.2% (w/w). NaAc at levels below 20 mmol/L promoted cell growth in the first six days, but the growth slowed on the seventh day when the NaAc concentration exceeded 15 mmol/L. The PHB content in the final biomass reached 11.0% of the dry cellular weight in the presence of 20 mmol/L NaAc. Two adjacent open reading frames (ORFs) in the genome of Synechocystis sp. PCC 6803, slr1993 and slr1994, were assigned to phbA and phbB, respectively, while the phbC gene was found to be far from these genes. This may account for the low expression of PHB in cyanobacteria.Synechocystis sp. PCC 6803 cell growth and poly β hydroxybutyrate (PHB) biosynthesis were studied in the presence of sodium acetate (NaAc). For nitrogen sufficient conditions, 15 mmol/L NaAc improved the PHB content up to 9.9% (w/w) while for nitrogen starved conditions, the PHB content was up to 15.2% (w/w). NaAc at levels below 20 mmol/L promoted cell growth in the first six days, but the growth slowed on the seventh day when the NaAc concentration exceeded 15 mmol/L. The PHB content in the final biomass reached 11.0% of the dry cellular weight in the presence of 20 mmol/L NaAc. Two adjacent open reading frames (ORFs) in the genome of Synechocystis sp. PCC 6803, slr1993 and slr1994, were assigned to phbA and phbB, respectively, while the phbC gene was found to be far from these genes. This may account for the low expression of PHB in cyanobacteria.

关 键 词：Synechocystis sp. PCC 6803 poly  β  hydroxybutyrate (PHB) ACETATE nitrogen starvation 

分 类 号：O632[理学—高分子化学]