Construction of cDNA library from iron-deficiency induced maize roots and screening and identification of iron-stress gene Fdr3  

作　　者：YIN Liping QI Xiaoting LIU Xianglin HUO Chunyan BAI Yanxia QIU Zesheng ZHANG Fusuo 

机构地区：[1]Department of Biology,Capital Normal University,Beijing 100037,China [2]China Agricultural University,Beijing 100094,China

出　　处：《Chinese Science Bulletin》2000年第12期1127-1131,共5页

摘　　要：To isolate Fe-deficient related (Fdr) genes, an expression cDNA library of 4.5×105 pfu/μg has been constructed from maize roots in iron-stress. 6 clones have been screened from the cDNA library by differential hybridization screening. It is proved that an Fdr3 cDNA clone expressed stronger under iron-deficient condition than under iron-sufficient one by Northern blot and Western blot.To isolate Fe-deficient related (Fdr) genes, an expression cDNA library of 4.5×105 pfu/μg has been constructed from maize roots in iron-stress. 6 clones have been screened from the cDNA library by differential hybridization screening. It is proved that anFdr3 cDNA clone expressed stronger under iron-deficient condition than under iron-sufficient one by Northern blot and Western blot.

关 键 词：maize iron-deficient cDNA library differential hybridization SCREENING iron-deficient related (fdr) clone. 

分 类 号：S513[农业科学—作物学]