Cloning of lea cDNA fragment. of carrot (Daucus carota L.) and analysis of its expression features  

作　　者：QI Mei CHEN Fan HUANG Meijuan WU Naihu 

机构地区：[1]Institute of Developmental Biology,Chinese Academy of Sciences,Beijing 100080,China [2]College of Life Sciences,Peking University,Beijing 100871,China

出　　处：《Chinese Science Bulletin》2000年第2期156-160,共5页

摘　　要：Addition of concentrated sucrose to MS culture arrests the development of. carrot somatic embryo at the stage of cotyledon embryo and, with the sucrose concentration restored to normal level, the embryo thus arrested is reactivated into post-embryonic development. Using the mathod of RT-PCR, the cDNA fragment of a new member of the Dc3 family of lea has been obtained from carrot somatic embryo under regulated state. As revealed by Northern blotting, strong expression has been observed in carrot somatic embryo unber regulated state but the expression was much reduced 12 h after deregulation, and nearly disappeared 24 h after. Based on this finding as well as results of related studies, it is surmised that changing the sucrose concentration in culture enabled carrot somatic embryo under suspension culture to undergo a specific course of development which is compabole to the dormancy-germination process of seeds.Addition of concentrated sucrose to MS culture arrests the development of carrot somatic embryo at the stage of cotyledon embryo and, with the sucrose concentration restored to normal level, the embryo thus arrested is reactivated into post-embryonic development. Using the method of RT-PCR, the cDNA fragment of a new member of the Dc3 family of lea has been obtained from carrot somatic embryo under regulated state. As revealed by Northern blotting, strong expression has been observed in carrot somatic embryo under regulated state but the expression was much reduced 12 h after deregulation, and nearly disappeared 24 h after. Based on this finding as well as results of related studies, it is surmised that changing the sucrose concentration in culture enabled carrot somatic embryo under suspension culture to undergo a specific course of development which is comparable to the dormancy-germination process of seeds.

关 键 词：CARROT SOMATIC EMBRYO regulated system LEA expression control. 

分 类 号：Q785[生物学—分子生物学] Q786