Trichloroacetate affects the EPR Signal Ⅱ_(slow) and Signal Ⅰ in the thylakoid of Chlamydomonas reinhardtii  

作　　者：YU Yong YU Xinjian YE Jiyu XU Chunhe Govindjee 

机构地区：[1]Shanghai Institute of Plant Physiology,Chinese Academy of Sciences,Shanghai 200032,China [2]Center of Biophysics&Computational Biology,University of Illinois,Urbana,Illinois 61801-3707.USA

出　　处：《Chinese Science Bulletin》2000年第23期2162-2168,共7页

基　　金：This work was supported by the State Key Bausic Research and Developmen Plan(Grant No.G1998010100);the National Natural Science Foundation of China(Grant No.39730040).

摘　　要：One electron paramagnetic resonance(EPR)signal,named SignalⅡslow,originates from the oxidized Tyrosine 160(Y_(D))of D2 polypeptide of photosystemⅡreaction center.After adding high concentration trichloroacetate(TCA)to the Chlamydomonas reinhardtii thylakoid suspension.this signal was abolished in a minute.Treatment of TCA also removes a few of polypeptides,including three extrinsic polypeptides of oxygen-evolving complex,from the thylakoid membrane.Based upon the analysis of the microenvironment around YD with a three-dimensional model,it is indicated that relatively high hydrophobicity of this microenvironment may be the essential prerequisite for TCA to affect YD.It has been observed that TCA treatment also retards the decay of the Signal I,produced by the oxidized reaction center chlorophyll dimer(P700^(+))of photosys-tem I.

关 键 词：THYLAKOID Y_(D) EPR trichloroacetate (TCA) three-dimensional model. 

分 类 号：Q945[生物学—植物学]