Construction of a genetic map and location of quantitative trait loci for dwarf trait in maize by RFLP markers  

作　　者：CAO Yongguo WANG Guoying WANG Shoucai WEI Yanling LU Jiang XIE Youju DAI Jingrui 

机构地区：[1]College of Plant Science and Technology,Chinese Agricultural University,Beijing 100094,China [2]National Laboratories for Agrobiotechnology,Chinese Agricultural Univerysity,Beijing 100094,China [3]Florida A&M University,Tallahassee,FL 32307,USA

出　　处：《Chinese Science Bulletin》2000年第3期247-250,共4页

摘　　要：Using F2 population derived from the cross of tall inbred 7922 by dwarf inbred 5003, an RFLP linkage map of maize has been constructed, on which 85 markers are distributed among 10 linkage groups and span maize genome about 1827.8 cM with an average distance (24.4 cM) between markers. 106 F2:3 lines of the population were grown in a 10×11 simple rectangular lattice design of one-raw plots with two replications and evaluated for plant height (PH). With interval mapping procedure, 5 QTLs controlling plant height have been identified and their genetic effects and gene action determined. 2 major OTLs with opposite effect have been discovered. One for increasing plant height is ph 1 which is located at chromosome 2 and accounts for 51.8% of the total phenotypic variation; the other for decreasing plant height is ph3 which is located at chromosome 5 and accounts for 38.6% of the total phenotypic variation. The chromosomal location of ph3 might be the same as or close to the position of bv1, a dwarf mutantUsing F2 population derived from the cross of tall inbred 7922 by dwarf inbred 5003, an RFLP linkage map of maize has been constructed, on which 85 markers are distributed among 10 linkage groups and span maize genome about 1827.8 cM with an average distance (24.4 cM) between markers. 106 F2:3 lines of the population were grown in a 10 × 11 simple rectangular lattice design of one-raw plots with two replications and evaluated for plant height (PH). With interval mapping procedure, 5 QTLs controlling plant height have been identified and their genetic effects and gene action determined. 2 major QTLs with opposite effect have been discovered. One for increasing plant height isph1 which is located at chromosome 2 and accounts for 51.8% of the total phenotypic variation; the other for decreasing plant height isph3 which is located at chromosome 5 and accounts for 38.6% of the total phenotypic variation. The chromosomal location ofph3 might be the same as or close to the position ofbv1, a dwarf mutant of maize.

关 键 词：ZEA mays L. RFLP genetic map DWARF TRAIT major QTL. 

分 类 号：S513[农业科学—作物学]