Immunochemical identification of gelsolin in maize pollen  

作　　者：Wei Wu Longfei Yan 

机构地区：[1]China Agr Univ, Coll Biol Sci, Minist Agr, Key Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China

出　　处：《Chinese Science Bulletin》2000年第3期256-259,共4页

摘　　要：Gelsolin is a representative of a type of actin-binding proteins (ABPs) universally found in eukaryotes. It plays role in nucleation, capping and severing of actin filaments in vitro. in our experiment, gelsolin was purified from pig plasma and the polycional antibodies against it were prepared. The crude extracts of maize pollen were immunodetected by western-blotting with polycional antibody and monoclonal antibody respectively. The immunodetection results show that gelsolin exists in maize pollen and its molecular weight is about 91 ku, similar to that of gelsolin found in animal tissues.Gelsolin is a representative of a type of actin-binding proteins (ABPs) universally found in eukaryotes. It plays role in nucleation, capping and severing of actin filamentsin vitro. In our experiment, gelsolin was purified from pig plasma and the polyclonal antibodies against it were prepared. The crude extracts of maize pollen were immunodetected by Western-blotting with polyclonal antibody and monoclonal antibody respectively. The immunodetection results show that gelsolin exists in maize pollen and its molecular weight is about 91 ku, similar to that of gelsolin found in animal tissues.

关 键 词：GELSOLIN POLLEN Western-blotting. 

分 类 号：S513[农业科学—作物学]