Expression of self-incompatibility ribonucleases of Antirrhinum in Escherichia coli  

作　　者：Huijun Yang Yongbiao Xue 

机构地区：[1]Chinese Acad Sci, Inst Dev Biol, Lab Plant & Dev Biol, Beijing 100080, Peoples R China

出　　处：《Chinese Science Bulletin》2000年第6期512-515,共4页

摘　　要：Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization inthe flowering plants. In many species, self-incompatibility is controlled by a single S locus with multiple alleles. So far, the only gene known in the S locus of the Solanaceae, Scrophulariaceae and Rosaceae encodes a class of ribonucleases, called self-incompatibility ribonucleases (S RNases), which have been shown to mediate stylar expression of self-incompatible reaction. As the first step to investigate their three-dimensional structure, we successfully expressed three biologically active S RNases of Antirrihnum (S2, S4 and S5) in Escherichia coli (E. coli). Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins. Possible reasons for non-lethality of S RNases on E. coliare discussed.Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization in the flowering plants. In many species, self-incompatibility is controlled by a single S locus with multiple alleles. So far, the only gene known in theS locus of the Solanaceae, Scrophulariaceae and Rosaceae encodes a class of ribonucleases, called self-incompatibility ribonucleases (S RNases), which have been shown to mediate stylar expression of self-incompatible reaction. As the first step to investigate their three-dimensional structure, we successfully expressed three biologically active S RNases of Antirrihnum (S 2,S 4 andS 5) inEscherichia coli (E. coli). Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins. Possible reasons for non-lethality of S RNases onE. coli are discussed.

关 键 词：S RIBONUCLEASES ANTIRRHINUM ESCHERICHIA coli. 

分 类 号：Q946[生物学—植物学]